Please use this identifier to cite or link to this item: https://doi.org/10.1021/la802672b
DC FieldValue
dc.titleOptical imaging of surface-immobilized oligonucleotide probes on DNA microarrays using liquid crystals
dc.contributor.authorLai, S.L.
dc.contributor.authorHuang, S.
dc.contributor.authorBi, X.
dc.contributor.authorYang, K.-L.
dc.date.accessioned2014-06-17T07:46:01Z
dc.date.available2014-06-17T07:46:01Z
dc.date.issued2009-01-06
dc.identifier.citationLai, S.L., Huang, S., Bi, X., Yang, K.-L. (2009-01-06). Optical imaging of surface-immobilized oligonucleotide probes on DNA microarrays using liquid crystals. Langmuir 25 (1) : 311-316. ScholarBank@NUS Repository. https://doi.org/10.1021/la802672b
dc.identifier.issn07437463
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/64338
dc.description.abstractIn this paper, we report a new label-free method for the imaging of immobilized oligonucleotide probes on DNA microarrays. The imaging principle is based on the disruption of orientations of nematic liquid crystals (LCs), 4-cyano- 4'-pentylbiphenyl (5CB), by the immobilized oligonucleotides on a surface. Because LCs are birefringent materials, disruption of their orientations by the immobilized oligonucleotides can manifest as optical signals visible to the naked eye. LC cells with two homeotropic boundary conditions, which align 5CB perpendicularly to both surfaces, were developed to deliver a distinct contrast between a dark background and a bright image caused by the immobilized oligonucleotides. This design also allows the quantification of immobilized oligonucleotide concentrations through the interference colors of LCs. The LC-based imaging method has a good signal-to-noise ratio and a clear distinction between positive and negative results and is nondestructive to the immobilized oligonucleotides. These advantages make it a promising means of assessing the quality of DNA microarrays. © 2009 American Chemical Society.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1021/la802672b
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.description.doi10.1021/la802672b
dc.description.sourcetitleLangmuir
dc.description.volume25
dc.description.issue1
dc.description.page311-316
dc.description.codenLANGD
dc.identifier.isiut000262176600056
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

39
checked on Oct 21, 2020

WEB OF SCIENCETM
Citations

39
checked on Oct 21, 2020

Page view(s)

64
checked on Oct 5, 2020

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.