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Title: Liquid crystal droplets as a hosting and sensing platform for developing immunoassays
Authors: Aliño, V.J.
Pang, J.
Yang, K.-L. 
Issue Date: 4-Oct-2011
Citation: Aliño, V.J., Pang, J., Yang, K.-L. (2011-10-04). Liquid crystal droplets as a hosting and sensing platform for developing immunoassays. Langmuir 27 (19) : 11784-11789. ScholarBank@NUS Repository.
Abstract: In this paper, we report an immunoassay in which probe proteins are immobilized on the surface of liquid crystal (LC) droplets rather than on solid surfaces. The advantage of this immunoassay is that the binding of antibodies to the probe proteins can be transduced by the LC droplets directly without the need for additional steps. For example, when we incubate the LC droplets decorated with immunoglobulin G (IgG) in a solution containing anti-IgG (AIgG), these droplets change their orientations from radial to bipolar configuration. In contrast, when we incubate the IgG-LC droplets in a solution containing anti-human serum albumin (AHSA), no changes are observed. The change of orientational configuration indicates the formation of the antigen-antibody immunocomplex on the surface of the LC droplets. Using LC droplet immunoassays, we successfully detect antibody concentrations as low as 0.01 μg/mL for AIgG and 0.02 μg/mL for AHSA. Because the immunoassay using LC droplets is label-free and gives a unique optical response, it has the potential to be further developed as a portable and low-cost immunoassay. © 2011 American Chemical Society.
Source Title: Langmuir
ISSN: 07437463
DOI: 10.1021/la2022215
Appears in Collections:Staff Publications

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