Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biomaterials.2011.04.023
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dc.titleGenomic instability of gold nanoparticle treated human lung fibroblast cells
dc.contributor.authorLi, J.J.
dc.contributor.authorLo, S.-L.
dc.contributor.authorNg, C.-T.
dc.contributor.authorGurung, R.L.
dc.contributor.authorHartono, D.
dc.contributor.authorHande, M.P.
dc.contributor.authorOng, C.-N.
dc.contributor.authorBay, B.-H.
dc.contributor.authorYung, L.Y.L.
dc.date.accessioned2014-06-17T07:41:48Z
dc.date.available2014-06-17T07:41:48Z
dc.date.issued2011-08
dc.identifier.citationLi, J.J., Lo, S.-L., Ng, C.-T., Gurung, R.L., Hartono, D., Hande, M.P., Ong, C.-N., Bay, B.-H., Yung, L.Y.L. (2011-08). Genomic instability of gold nanoparticle treated human lung fibroblast cells. Biomaterials 32 (23) : 5515-5523. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2011.04.023
dc.identifier.issn01429612
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/63976
dc.description.abstractGold nanoparticles (AuNPs) are one of the most versatile and widely researched materials for novel biomedical applications. However, the current knowledge in their toxicological profile is still incomplete and many on-going investigations aim to understand the potential adverse effects in human body. Here, we employed two dimensional gel electrophoresis to perform a comparative proteomic analysis of AuNP treated MRC-5 lung fibroblast cells. In our findings, we identified 16 proteins that were differentially expressed in MRC-5 lung fibroblasts following exposure to AuNPs. Their expression levels were also verified by western blotting and real time RT-PCR analysis. Of interest was the difference in the oxidative stress related proteins (NADH ubiquinone oxidoreductase (NDUFS1), protein disulfide isomerase associate 3 (PDIA3), heterogeneous nuclear ribonucleus protein C1/C2 (hnRNP C1/C2) and thioredoxin-like protein 1 (TXNL1)) as well as proteins associated with cell cycle regulation, cytoskeleton and DNA repair (heterogeneous nuclear ribonucleus protein C1/C2 (hnRNP C1/C2) and Secernin-1 (SCN1)). This finding is consistent with the genotoxicity observed in the AuNP treated lung fibroblasts. These results suggest that AuNP treatment can induce oxidative stress-mediated genomic instability. © 2011 Elsevier Ltd.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.biomaterials.2011.04.023
dc.sourceScopus
dc.subjectGenotoxicity
dc.subjectGold nanoparticles
dc.subjectLung fibroblast
dc.subjectProteomic analysis
dc.typeArticle
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.contributor.departmentEPIDEMIOLOGY & PUBLIC HEALTH
dc.description.doi10.1016/j.biomaterials.2011.04.023
dc.description.sourcetitleBiomaterials
dc.description.volume32
dc.description.issue23
dc.description.page5515-5523
dc.description.codenBIMAD
dc.identifier.isiut000291900900021
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