Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/63943
DC FieldValue
dc.titleForce measurements of bacterial adhesion on metals using a cell probe atomic force microscope
dc.contributor.authorSheng, X.
dc.contributor.authorTing, Y.P.
dc.contributor.authorPehkonen, S.O.
dc.date.accessioned2014-06-17T07:41:22Z
dc.date.available2014-06-17T07:41:22Z
dc.date.issued2007-06-15
dc.identifier.citationSheng, X., Ting, Y.P., Pehkonen, S.O. (2007-06-15). Force measurements of bacterial adhesion on metals using a cell probe atomic force microscope. Journal of Colloid and Interface Science 310 (2) : 661-669. ScholarBank@NUS Repository.
dc.identifier.issn00219797
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/63943
dc.description.abstractThe adhesion of microbial cells to metal surfaces in aqueous media is an important phenomenon in both the natural environment and engineering systems. The adhesion of two anaerobic sulfate-reducing bacteria (Desulfovibrio desulfuricans and a local marine isolate) and an aerobe (Pseudomonas sp.) to four polished metal surfaces (i.e., stainless steel 316, mild steel, aluminum, and copper) was examined using a force spectroscopy technique with an atomic force microscope (AFM). Using a modified bacterial tip, the attraction and repulsion forces (in the nano-Newton range) between the bacterial cell and the metal surface in aqueous media were quantified. Results show that the bacterial adhesion force to aluminum is the highest among the metals investigated, whereas the one to copper is the lowest. The bacterial adhesion forces to metals are influenced by both the electrostatic force and metal surface hydrophobicity. It is also found that the physiological properties of the bacterium, namely the bacterial surface charges and hydrophobicity, also have influence on the bacteria-metal interaction. The adhesion to the metals by Pseudomonas sp. and D. desulfuricans was greater than by the marine SRB isolate. The cell-cell interactions show that there are strong electrostatic repulsion forces between bacterial cells. Cell probe atomic force microscopy has provided some useful insight into the interactions of bacterial cells with the metal surfaces. © 2007 Elsevier Inc. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.jcis.2007.01.084
dc.sourceScopus
dc.subjectAdhesion force
dc.subjectAtomic force microscopy
dc.subjectCell probe
dc.subjectDesulfovibrio desulfuricans
dc.subjectPseudomonas sp.
dc.subjectSulfate-reducing bacteria
dc.typeArticle
dc.contributor.departmentDIVISION OF ENVIRONMENTAL SCIENCE & ENGG
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.description.sourcetitleJournal of Colloid and Interface Science
dc.description.volume310
dc.description.issue2
dc.description.page661-669
dc.description.codenJCISA
dc.identifier.isiut000246459700038
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

62
checked on Mar 19, 2018

WEB OF SCIENCETM
Citations

64
checked on Dec 19, 2018

Page view(s)

48
checked on Dec 1, 2019

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.