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https://doi.org/10.1039/c2md20240a
Title: | Fluorescence turn-on detection of live cell apoptosis using a hyperbranched conjugated polyelectrolyte | Authors: | Shi, H. Wang, G. Liang, J. Liu, B. |
Issue Date: | Mar-2013 | Citation: | Shi, H., Wang, G., Liang, J., Liu, B. (2013-03). Fluorescence turn-on detection of live cell apoptosis using a hyperbranched conjugated polyelectrolyte. MedChemComm 4 (3) : 554-558. ScholarBank@NUS Repository. https://doi.org/10.1039/c2md20240a | Abstract: | A fluorescence "turn-on" assay for live cell apoptosis imaging is developed based on self-assembled complexes between a cationic water-soluble hyperbranched conjugated polyelectrolyte (HCPE) and an Asp-Glu-Val-Asp (DEVD) peptide substrate labelled with a fluorescence quencher (DEVD-dabcyl). The polymer fluorescence can be significantly quenched upon DEVD-dabcyl binding, and the fluorescence is switched on upon treatment of the complexes with caspase-3/7, which are activated in the cell apoptotic process and are able to cleave the DEVD moiety. The light-up nature of the assay allows monitoring of caspase-3/7 activities both in solutions and in living cells with a high signal to noise ratio, which provides a new opportunity to screen caspase inhibitors and detect cell apoptosis. © 2013 The Royal Society of Chemistry. | Source Title: | MedChemComm | URI: | http://scholarbank.nus.edu.sg/handle/10635/63930 | ISSN: | 20402503 | DOI: | 10.1039/c2md20240a |
Appears in Collections: | Staff Publications |
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