Please use this identifier to cite or link to this item: https://doi.org/10.1021/la2004694
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dc.titleEnhancing the fluorescence intensity of DNA microarrays by using cationic surfactants
dc.contributor.authorLai, S.L.
dc.contributor.authorChen, C.-H.
dc.contributor.authorYang, K.-L.
dc.date.accessioned2014-06-17T07:40:19Z
dc.date.available2014-06-17T07:40:19Z
dc.date.issued2011-05-03
dc.identifier.citationLai, S.L., Chen, C.-H., Yang, K.-L. (2011-05-03). Enhancing the fluorescence intensity of DNA microarrays by using cationic surfactants. Langmuir 27 (9) : 5659-5664. ScholarBank@NUS Repository. https://doi.org/10.1021/la2004694
dc.identifier.issn07437463
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/63851
dc.description.abstractDNA microarrays have been used as powerful tools in genomics studies and single nucleotide polymorphisms analysis. However, the fluorescence detection used in most conventional DNA microarrays is still limited by its sensitivity. The aim of this study is to use a cationic surfactant, cetyl trimethylammonium bromide (CTAB), to enhance the fluorescence intensity of 6-carboxy-fluorescene (FAM)-labeled DNA probes immobilized on a DNA microarray. We show that in the presence of CTAB the immobilized FAM-labeled DNA probes is 11-fold brighter than that without exposure to CTAB. Similarly, when we hybridize FAM-labeled DNA targets to a DNA microarray and treat the surface with CTAB solution, the fluorescence intensity shows a 26-fold increase for perfect-match DNA targets. More importantly, the contrast between perfect-match and 1-mismatch DNA is also increased from 1.3-fold to 15-fold. This method offers a simple and efficient technique to enhance the detection limit of DNA microarrays. © 2011 American Chemical Society.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1021/la2004694
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.description.doi10.1021/la2004694
dc.description.sourcetitleLangmuir
dc.description.volume27
dc.description.issue9
dc.description.page5659-5664
dc.description.codenLANGD
dc.identifier.isiut000289742500060
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