Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biortech.2012.07.103
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dc.titleEfficient production of biodiesel from waste grease: One-pot esterification and transesterification with tandem lipases
dc.contributor.authorYan, J.
dc.contributor.authorLi, A.
dc.contributor.authorXu, Y.
dc.contributor.authorNgo, T.P.N.
dc.contributor.authorPhua, S.
dc.contributor.authorLi, Z.
dc.date.accessioned2014-06-17T07:39:42Z
dc.date.available2014-06-17T07:39:42Z
dc.date.issued2012-11
dc.identifier.citationYan, J., Li, A., Xu, Y., Ngo, T.P.N., Phua, S., Li, Z. (2012-11). Efficient production of biodiesel from waste grease: One-pot esterification and transesterification with tandem lipases. Bioresource Technology 123 : 332-337. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biortech.2012.07.103
dc.identifier.issn09608524
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/63801
dc.description.abstractA novel concept and efficient method for producing biodiesel (FAME) from grease (15-40. wt% free fatty acid, FFA) were developed by using tandem lipases for one-pot esterification of FFA and transesterification of triglyceride with methanol in a solvent-free system. Combining immobilized Candida antarctica lipase B (CALB) (Novozyme 435) favoring the esterification and immobilized Thermomyces lanuginosus lipase (TLL) (Lipozyme TLIM) preferring the transesterification at 2:8 (wt/wt) gave FAME in 80% yield, being better than that with Novozyme 435 or Lipozyme TLIM. Recombinant Escherichia coli (Calb/Tll) co-expressing CALB and TLL was engineered as a more efficient tandem-lipases system. Using wet or dry cells (4. wt%) gave FAME in 87% or 95% yield, which is much better than that with E. coli cells expressing either CALB or TLL alone. Cells of E. coli (Calb/Tll) were recycled for five times and retained 75% productivity, thus being practical for producing biodiesel from grease. © 2012 Elsevier Ltd.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.biortech.2012.07.103
dc.sourceScopus
dc.subjectBiodiesel
dc.subjectFree fatty acid
dc.subjectGrease
dc.subjectTandem enzymes
dc.subjectWhole-cells catalysis
dc.typeArticle
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.description.doi10.1016/j.biortech.2012.07.103
dc.description.sourcetitleBioresource Technology
dc.description.volume123
dc.description.page332-337
dc.description.codenBIRTE
dc.identifier.isiut000310401100049
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