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Title: Development of a chemically defined minimal medium for the exponential growth of Leuconostoc mesenteroides ATCC8293
Authors: Kim, Y.J.
Eom, H.-J.
Seo, E.-Y.
Lee, D.Y. 
Kim, J.H.
Han, N.S.
Keywords: Cell growth rate
Chemically defined minimal medium
Leuconostoc mesenteroides
Single omission technique
Issue Date: Nov-2012
Citation: Kim, Y.J., Eom, H.-J., Seo, E.-Y., Lee, D.Y., Kim, J.H., Han, N.S. (2012-11). Development of a chemically defined minimal medium for the exponential growth of Leuconostoc mesenteroides ATCC8293. Journal of Microbiology and Biotechnology 22 (11) : 1518-1522. ScholarBank@NUS Repository.
Abstract: Leuconostoc mesenteroides is a heterofermentative Gram-positive bacterium that plays key roles in fermentation of foods such as kimchi, sauerkraut, and milk, leading to the production of various organic acids and aromatic compounds. To study the microbiological and genomic characteristics of L. mesenteroides, we have developed a new chemically defined minimal medium by using the single omission technique. During the exponential cell growth, this species required glutamine, methionine, valine, and nicotinic acid as essential nutrients and 8 amino acids (arginine, cysteine, histidine, leucine, phenylalanine, proline, threonine, and tryptophan), 5 vitamins (ascorbic acid, folic acid, inosine, calcium panthothenate, and thiamine), and others (manganese, magnesium, adenine, uracil, and Tween 80) as supplemental nutrients. This medium is useful to study the metabolic characteristics of L. mesenteroides and to explain its role in food fermentation. © The Korean Society for Microbiology and Biotechnology.
Source Title: Journal of Microbiology and Biotechnology
ISSN: 10177825
DOI: 10.4014/jmb.1205.05053
Appears in Collections:Staff Publications

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