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Title: Cobalt chromium alloy with immobilized BMP peptide for enhanced bone growth
Authors: Poh, C.K.
Shi, Z. 
Tan, X.W.
Liang, Z.C.
Foo, X.M.
Tan, H.C.
Neoh, K.G. 
Wang, W.
Keywords: BMP peptide
cobalt chromium
orthopedic implants
surface modification
Issue Date: Sep-2011
Citation: Poh, C.K., Shi, Z., Tan, X.W., Liang, Z.C., Foo, X.M., Tan, H.C., Neoh, K.G., Wang, W. (2011-09). Cobalt chromium alloy with immobilized BMP peptide for enhanced bone growth. Journal of Orthopaedic Research 29 (9) : 1424-1430. ScholarBank@NUS Repository.
Abstract: Cobalt chromium (CoCr) alloys are widely used in orthopedic practice, however, lack of integration into the bone for long-term survival often occurs, leading to implant failure. Revision surgery to address such a failure involves increased risks, complications, and costs. Advances to enhancement of bone-implant interactions would improve implant longevity and long-term results. Therefore, we investigated the effects of BMP peptide covalently grafted to CoCr alloy on osteogenesis. The BMP peptide was derived from the knuckle epitope of bone morphogenic protein-2 (BMP-2) and was conjugated via a cysteine amino acid at the N-terminus. X-ray photoelectron spectroscopy and o-phthaldialdehyde were used to verify successful grafting at various stages of surface functionalization. Surface topography was evaluated from the surface profile determined by atomic force microscopy. Osteoblastic cells (MC3T3-E1) were seeded on the substrates, and the effects of BMP peptide on osteogenic differentiation were evaluated by measuring alkaline phosphatase (ALP) activity and calcium mineral deposition. The functionalized surfaces showed a twofold increase in ALP activity after 2 weeks incubation and a fourfold increase in calcium content after 3 weeks incubation compared to the pristine substrate. These findings are potentially useful in the development of improved CoCr implants for use in orthopedic applications. © 2011 Orthopaedic Research Society.
Source Title: Journal of Orthopaedic Research
ISSN: 07360266
DOI: 10.1002/jor.21409
Appears in Collections:Staff Publications

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