Please use this identifier to cite or link to this item:
Title: Chemical modification of P84 polyimide as anion-exchange membranes in a free-flow isoelectric focusing system for protein separation
Authors: Cheng, J.-H. 
Xiao, Y.-C. 
Wu, C.
Chung, T.-S. 
Keywords: Electrophoresis
Ion exchange membrane
Ion exchange membrane partitioned free-flow isoelectric focusing (IEM-FFIEF)
pH gradient
Protein separation
Issue Date: 15-May-2010
Citation: Cheng, J.-H., Xiao, Y.-C., Wu, C., Chung, T.-S. (2010-05-15). Chemical modification of P84 polyimide as anion-exchange membranes in a free-flow isoelectric focusing system for protein separation. Chemical Engineering Journal 160 (1) : 340-350. ScholarBank@NUS Repository.
Abstract: By means of amination with diamine and methylation with methyl iodide, we have modified P84 microporous polyimide membranes with characteristics of highly charged anion-exchange membranes. FTIR (Fourier transform infrared-attenuated total reflection) and XPS (X-ray photoelectron spectroscopy) confirm the amination and methylation reactions on membrane surfaces. The intrinsic properties of the newly developed anion-exchange membranes have been fully characterized in terms of ζ-potentials, electrical resistances, PWPs (pure water permeation) and pore size distributions. By using the newly developed membranes, a free-flow isoelectric focusing (IEM-FFIEF) has been set up for the separation of myoglobin (Mb) and lysozyme (Lys) mixtures. Experimental data show that (1) the Mb flux via the highly charged P84 anion-exchange membrane can be 10 times higher than that of the original P84 membrane and (2) the high surface charge is the predominant factor for the enhanced Mb flux. HPLC (high performance liquid chromatography) results show that not only the Mb flux was high, but also its purity in the permeate side is extremely high. It is therefore concluded that the diamine and methyl iodide modifications can effectively modulate P84 nano-porous membranes with anion-exchange characteristics, which is suitable for the IEM-FFIEF application. © 2010 Elsevier B.V. All rights reserved.
Source Title: Chemical Engineering Journal
ISSN: 13858947
DOI: 10.1016/j.cej.2010.02.058
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Sep 23, 2022


checked on Sep 23, 2022

Page view(s)

checked on Sep 22, 2022

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.