Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biomaterials.2008.10.029
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dc.titleBMP-2 plasmid loaded PLGA/HAp composite scaffolds for treatment of bone defects in nude mice
dc.contributor.authorNie, H.
dc.contributor.authorHo, M.-L.
dc.contributor.authorWang, C.-K.
dc.contributor.authorWang, C.-H.
dc.contributor.authorFu, Y.-C.
dc.date.accessioned2014-06-17T07:36:45Z
dc.date.available2014-06-17T07:36:45Z
dc.date.issued2009-02
dc.identifier.citationNie, H., Ho, M.-L., Wang, C.-K., Wang, C.-H., Fu, Y.-C. (2009-02). BMP-2 plasmid loaded PLGA/HAp composite scaffolds for treatment of bone defects in nude mice. Biomaterials 30 (5) : 892-901. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2008.10.029
dc.identifier.issn01429612
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/63549
dc.description.abstractWe studied three different types of scaffolds, encapsulating bone morphogenetic protein-2 (BMP-2) plasmid, in terms of their performances in bone regeneration in nude mice. The plasmid was loaded into fibrous matrices in three different ways: coating of naked DNA (Group A) or DNA/chitosan nanoparticles (Group B) onto scaffolds after fiber fabrication by dripping, and encapsulation of DNA/chitosan nanoparticles into scaffold by mixing them with PLGA/DCM solution before fiber fabrication (Group C). Their individual performances were examined by soft X-ray observation, histological analysis and immunostaining of bone tissue. In addition, the BMP-2 protein concentration and alkaline phosphatase (ALP) activity in serum were monitored. The results revealed that the bioactivity of BMP-2 plasmid released from all three kinds of scaffolds was well maintained; this eventually helped improve the healing of segmental defects in vivo. Interestingly, the three kinds of scaffolds released DNA or DNA nanoparticles in different modes and their performances in bone healing were diverse. These observations demonstrate that the in vivo performance of these newly developed DNA delivery devices correlates well with their in vitro release profiles. © 2008 Elsevier Ltd. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.biomaterials.2008.10.029
dc.sourceScopus
dc.subjectBone regeneration
dc.subjectDNA delivery
dc.subjectElectrospinning
dc.subjectHydroxyapatite
dc.subjectNude mice
dc.subjectPLGA
dc.typeArticle
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.description.doi10.1016/j.biomaterials.2008.10.029
dc.description.sourcetitleBiomaterials
dc.description.volume30
dc.description.issue5
dc.description.page892-901
dc.description.codenBIMAD
dc.identifier.isiut000262552600024
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