Please use this identifier to cite or link to this item:
|dc.title||BMP-2 plasmid loaded PLGA/HAp composite scaffolds for treatment of bone defects in nude mice|
|dc.identifier.citation||Nie, H., Ho, M.-L., Wang, C.-K., Wang, C.-H., Fu, Y.-C. (2009-02). BMP-2 plasmid loaded PLGA/HAp composite scaffolds for treatment of bone defects in nude mice. Biomaterials 30 (5) : 892-901. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biomaterials.2008.10.029|
|dc.description.abstract||We studied three different types of scaffolds, encapsulating bone morphogenetic protein-2 (BMP-2) plasmid, in terms of their performances in bone regeneration in nude mice. The plasmid was loaded into fibrous matrices in three different ways: coating of naked DNA (Group A) or DNA/chitosan nanoparticles (Group B) onto scaffolds after fiber fabrication by dripping, and encapsulation of DNA/chitosan nanoparticles into scaffold by mixing them with PLGA/DCM solution before fiber fabrication (Group C). Their individual performances were examined by soft X-ray observation, histological analysis and immunostaining of bone tissue. In addition, the BMP-2 protein concentration and alkaline phosphatase (ALP) activity in serum were monitored. The results revealed that the bioactivity of BMP-2 plasmid released from all three kinds of scaffolds was well maintained; this eventually helped improve the healing of segmental defects in vivo. Interestingly, the three kinds of scaffolds released DNA or DNA nanoparticles in different modes and their performances in bone healing were diverse. These observations demonstrate that the in vivo performance of these newly developed DNA delivery devices correlates well with their in vitro release profiles. © 2008 Elsevier Ltd. All rights reserved.|
|dc.contributor.department||CHEMICAL & BIOMOLECULAR ENGINEERING|
|Appears in Collections:||Staff Publications|
Show simple item record
Files in This Item:
There are no files associated with this item.
checked on Jan 23, 2020
WEB OF SCIENCETM
checked on Jan 15, 2020
checked on Dec 29, 2019
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.