Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.jcis.2013.05.060
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dc.titleAssessment of stability of surface anchors for antibacterial coatings and immobilized growth factors on titanium
dc.contributor.authorZheng, D.
dc.contributor.authorNeoh, K.G.
dc.contributor.authorShi, Z.
dc.contributor.authorKang, E.-T.
dc.date.accessioned2014-06-17T07:36:23Z
dc.date.available2014-06-17T07:36:23Z
dc.date.issued2013-09-15
dc.identifier.citationZheng, D., Neoh, K.G., Shi, Z., Kang, E.-T. (2013-09-15). Assessment of stability of surface anchors for antibacterial coatings and immobilized growth factors on titanium. Journal of Colloid and Interface Science 406 : 238-246. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jcis.2013.05.060
dc.identifier.issn00219797
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/63517
dc.description.abstractTitanium (Ti) has been functionalized with biomolecules for biomedical purposes. However, there is very limited information on the stability of such functionalities. Ti surface functionalized with carboxymethyl chitosan (CMCS) and bone morphogenetic protein 2 (BMP-2) has been reported to inhibit bacterial colonization while at the same time enhances osteoblast functions. In this work, three types of anchoring molecules, (3-aminopropyl) triethoxysilane (Silane), dopamine (DA), and polydopamine (PDA), were used for immobilizing the CMCS on Ti. The CMCS-modified surfaces were subjected to 70% ethanol treatment, autoclaving, and prolonged immersion in phosphate buffered saline (PBS). After the treatment procedures, the ability of the CMCS-modified substrates to inhibit colonization by Staphylococcus epidermidis (. S. epidermidis) was assessed to evaluate the stability of the immobilized CMCS. The bacterial adhesion assays showed that the CMCS-DA- and CMCS-PDA-modified Ti remained stable after 70% ethanol treatment, autoclaving, and prolonged immersion in PBS, whereas the CMCS-Silane-modified Ti was less stable after autoclaving and prolonged immersion in PBS. The CMCS-DA- and CMCS-PDA-modified Ti substrates were functionalized with BMP-2 and used to support osteoblast growth. Evaluation of alkaline phosphatase (ALP) activity and calcium deposition from osteoblasts cultured on these substrates, which have been treated with 70% ethanol, or subjected to autoclaving, and prolonged immersion in PBS indicated that the immobilized BMP-2 on these surfaces retained its bioactivity. © 2013 Elsevier Inc.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.jcis.2013.05.060
dc.sourceScopus
dc.subjectBacterial colonization
dc.subjectBMP-2
dc.subjectCarboxymethyl chitosan
dc.subjectFunctionalization
dc.subjectStability
dc.subjectTitanium
dc.typeArticle
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.description.doi10.1016/j.jcis.2013.05.060
dc.description.sourcetitleJournal of Colloid and Interface Science
dc.description.volume406
dc.description.page238-246
dc.description.codenJCISA
dc.identifier.isiut000322094300029
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