Please use this identifier to cite or link to this item:
|Title:||Three-dimensional (3D) extra-cellular matrix coating of a microfluidic device||Authors:||Chaw, K.C.
|Issue Date:||1-Apr-2006||Citation:||Chaw, K.C., Manimaran, M., Tay, F.E.H., Swaminathan, S. (2006-04-01). Three-dimensional (3D) extra-cellular matrix coating of a microfluidic device. Journal of Physics: Conference Series 34 (1) : 747-751. ScholarBank@NUS Repository. https://doi.org/10.1088/1742-6596/34/1/123||Abstract:||Cell migration on two-dimensional (2-D) surface is distinctively different from cell migration in threedimensional (3-D) matrix. Although cell motility on 2-D matrix is widely studied, 3-D cell migration is just beginning to be understood. With the development of micro-fluidic systems, it is possible to study cell motility under much better controlled conditions. In this work, we present techniques for controlled coating of 3-D extracellular matrix (ECM) within microfluidic devices. This coating technique relies on the dominance of capillary forces and surface tensions in micro systems. We have successfully created uniform coating of Matrigel across microgaps of different lengths. This simple and reproducible method will have widespread biological applications such as the study of 3-D cell migration, the coating of basement membrane for cancer metastasis and angiogenesis study. © 2006 IOP Publishing Ltd.||Source Title:||Journal of Physics: Conference Series||URI:||http://scholarbank.nus.edu.sg/handle/10635/61572||ISSN:||17426588||DOI:||10.1088/1742-6596/34/1/123|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.