Please use this identifier to cite or link to this item: https://doi.org/10.1080/17452750802551298
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dc.titleMechanical and in vitro evaluations of composite PLDLLA/TCP scaffolds for bone engineering
dc.contributor.authorLam, C.X.F.
dc.contributor.authorOlkowski, R.
dc.contributor.authorSwieszkowski, W.
dc.contributor.authorTan, K.C.
dc.contributor.authorGibson, I.
dc.contributor.authorHutmacher, D.W.
dc.date.accessioned2014-06-17T06:26:18Z
dc.date.available2014-06-17T06:26:18Z
dc.date.issued2008-12
dc.identifier.citationLam, C.X.F., Olkowski, R., Swieszkowski, W., Tan, K.C., Gibson, I., Hutmacher, D.W. (2008-12). Mechanical and in vitro evaluations of composite PLDLLA/TCP scaffolds for bone engineering. Virtual and Physical Prototyping 3 (4) : 193-197. ScholarBank@NUS Repository. https://doi.org/10.1080/17452750802551298
dc.identifier.issn17452759
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/60698
dc.description.abstractBone tissue engineering scaffolds have two challenging functional tasks to play; to be bioactive by encouraging cell proliferation and differentiation, and to provide suitable mechanical stability upon implantation. Composites of biopolymers and bioceramics unite the advantages of both materials, resulting in better processability, enhanced mechanical properties through matrix reinforcement and osteoinductivity. Novel composite blends of poly(L-lactide-co-D,L-lactide)/tricalcium phosphate (PLDLLA/TCP) were fabricated into scaffolds by an extrusion deposition technique customised from standard rapid prototyping technology. PLDLLA/TCP composite material blends of various compositions were prepared and analysed for their mechanical properties. PLDLLA/TCP (10%) was optimised and fabricated into scaffolds. Compressive mechanical properties for the composite scaffolds were measured. In vitro studies were conducted using porcine bone-marrow stromal cells (BMSCs). Cell-scaffold constructs were induced using osteogenic induction factors for up to 8 weeks. Cell proliferation, viability and differentiation capabilities were assayed using phase-contrast light microscopy, scanning electron microscopy, DNA quantification (Pico Green), Alamar Blue metabolic assay; FDA/PI fluorescent assay and western blot analysis for osteopontin. Microscopy observations showed BMSCs possessed high proliferative capabilities and demonstrated bridging across the pores of the scaffolds. FDA/PI staining as well as Alamar Blue assay showed high viability of BMSCs cultured on the composite scaffolds. Cell numbers, based on DNA quantitation, were observed to increase continuously up to the eighth week of study. Western blot analysis showed increased osteopontin synthesis on the scaffolds compared to tissue culture plastic. Based on our results the PLDLLA/TCP scaffolds exhibited good potential and biocompatibility for bone tissue engineering applications.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1080/17452750802551298
dc.sourceScopus
dc.subjectBioresorbable Scaffolds
dc.subjectBone
dc.subjectComposite
dc.subjectPLDLLA
dc.subjectTissue engineering
dc.typeArticle
dc.contributor.departmentBIOENGINEERING
dc.contributor.departmentMECHANICAL ENGINEERING
dc.description.doi10.1080/17452750802551298
dc.description.sourcetitleVirtual and Physical Prototyping
dc.description.volume3
dc.description.issue4
dc.description.page193-197
dc.identifier.isiut000213913800002
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