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Title: Electrospun nanofiber scaffolds for rapid and rich capture of bone marrow-derived hematopoietic stem cells
Authors: Ma, K.
Chan, C.K. 
Liao, S. 
Hwang, W.Y.K.
Feng, Q.
Ramakrishna, S. 
Keywords: Cell adhesion
Hematopoietic stem cells
Issue Date: May-2008
Citation: Ma, K., Chan, C.K., Liao, S., Hwang, W.Y.K., Feng, Q., Ramakrishna, S. (2008-05). Electrospun nanofiber scaffolds for rapid and rich capture of bone marrow-derived hematopoietic stem cells. Biomaterials 29 (13) : 2096-2103. ScholarBank@NUS Repository.
Abstract: Interactions between bone marrow-derived hematopoietic stem cells (BM-HSCs) and their local microenvironment are an integral part of signaling control of BM-HSCs migration, proliferation and differentiation. We hypothesized that both substrate topographical and biochemical cues promote BM-HSCs adhesive behaviors, which are crucial for BM-HSCs' homing, self-renewal and lineage commitment within their microenvironment. We employed electrospinning technique to fabricate nanofiber scaffolds (NFS) with poly(dl-lactide-co-glycolide) blended with collagen I. NFS was further coated with E-selectin, a critical adhesive biomolecule. Capture efficiency study showed that blended NFS, after coated with E-selectin, significantly increased cell capture percentage from 23.40% to 67.41% within 30 min and from 29.44% to 70.19% within 60 min of incubation at room temperature. This study highlights the potential of using a biomimetic scaffold to design a site-specific niche-like unit for facilitating proliferation or differentiation functions of BM-HSCs. © 2008 Elsevier Ltd. All rights reserved.
Source Title: Biomaterials
ISSN: 01429612
DOI: 10.1016/j.biomaterials.2008.01.024
Appears in Collections:Staff Publications

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