Please use this identifier to cite or link to this item: https://doi.org/10.1021/bm050743i
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dc.titleCoaxial electrospinning of (fluorescein isothiocyanate-conjugated bovine serum albumin)-encapsulated poly(ε-caprolactone) nanofibers for sustained release
dc.contributor.authorZhang, Y.Z.
dc.contributor.authorWang, X.
dc.contributor.authorFeng, Y.
dc.contributor.authorLi, J.
dc.contributor.authorLim, C.T.
dc.contributor.authorRamakrishna, S.
dc.date.accessioned2014-06-17T06:14:48Z
dc.date.available2014-06-17T06:14:48Z
dc.date.issued2006-04
dc.identifier.citationZhang, Y.Z., Wang, X., Feng, Y., Li, J., Lim, C.T., Ramakrishna, S. (2006-04). Coaxial electrospinning of (fluorescein isothiocyanate-conjugated bovine serum albumin)-encapsulated poly(ε-caprolactone) nanofibers for sustained release. Biomacromolecules 7 (4) : 1049-1057. ScholarBank@NUS Repository. https://doi.org/10.1021/bm050743i
dc.identifier.issn15257797
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/59718
dc.description.abstractAs an aim toward developing biologically mimetic and functional nanofiber-based tissue engineering scaffolds, we demonstrated the encapsulation of a model protein, fluorescein isothiocyanate-conjugated bovine serum albumin (fitcBSA), along with a water-soluble polymer, poly(ethylene glycol) (PEG), within the biodegradable poly(ε-caprolactone) (PCL) nanofibers using a coaxial electrospinning technique. By variation of the inner flow rates from 0.2 to 0.6 mL/h with a constant outer flow rate of 1.8 mL/h, fitcBSA loadings of 0.85-2.17 mg/g of nanofibrous membranes were prepared. Variation of flow rates also resulted in increases of fiber sizes from ca. 270 nm to 380 nm. The encapsulation of fitcBSA/PEG within PCL was subsequently characterized by laser confocal scanning microscopy, transmission electron microscopy (TEM), and X-ray photoelectron spectroscopy (XPS) analysis. In vitro release studies were conducted to evaluate sustained release potential of the core-sheath-structured composite nanofiber PCL-r-fitcBSA/PEG. As a negative control, composite nanofiber PCL/ fitcBSA/PEG blend was prepared from a normal electrospinning method. It was found that core-sheath nanofibers PCL-r-fitcBSA/PEG pronouncedly alleviated the initial burst release for higher protein loading and gave better sustainability compared to that of PCL/fitcBSA/PEG nanofibers. The present study would provide a basis for further design and optimization of processing conditions to control the nanostructure of core-sheath composite nanofibers and ultimately achieve desired release kinetics of bioactive proteins (e.g., growth factors) for practical tissue engineering applications. © 2006 American Chemical Society.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1021/bm050743i
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentPHYSICS
dc.contributor.departmentBIOENGINEERING
dc.contributor.departmentMECHANICAL ENGINEERING
dc.description.doi10.1021/bm050743i
dc.description.sourcetitleBiomacromolecules
dc.description.volume7
dc.description.issue4
dc.description.page1049-1057
dc.description.codenBOMAF
dc.identifier.isiut000236868800008
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