Please use this identifier to cite or link to this item: https://doi.org/10.1063/1.3671065
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dc.titleCharacterization and separation of Cryptosporidium and Giardia cells using on-chip dielectrophoresis
dc.contributor.authorNarayanan Unni, H.
dc.contributor.authorHartono, D.
dc.contributor.authorYue Lanry Yung, L.
dc.contributor.authorMah-Lee Ng, M.
dc.contributor.authorPueh Lee, H.
dc.contributor.authorCheong Khoo, B.
dc.contributor.authorLim, K.-M.
dc.date.accessioned2014-06-17T06:14:26Z
dc.date.available2014-06-17T06:14:26Z
dc.date.issued2012-03-02
dc.identifier.citationNarayanan Unni, H., Hartono, D., Yue Lanry Yung, L., Mah-Lee Ng, M., Pueh Lee, H., Cheong Khoo, B., Lim, K.-M. (2012-03-02). Characterization and separation of Cryptosporidium and Giardia cells using on-chip dielectrophoresis. Biomicrofluidics 6 (1) : -. ScholarBank@NUS Repository. https://doi.org/10.1063/1.3671065
dc.identifier.issn19321058
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/59689
dc.description.abstractDielectrophoresis (DEP) has been shown to have significant potential for the characterization of cells and could become an efficient tool for rapid identification and assessment of microorganisms. The present work is focused on the trapping, characterization, and separation of two species of Cryptosporidium (C. parvum and C. muris) and Giardia lambia (G. lambia) using a microfluidic experimental setup. Cryptosporidium oocysts, which are 2-4 μm in size and nearly spherical in shape, are used for the preliminary stage of prototype development and testing. G. lambia cysts are 8-12 μm in size. In order to facilitate effective trapping, simulations were performed to study the effects of buffer conductivity and applied voltage on the flow and cell transport inside the DEP chip. Microscopic experiments were performed using the fabricated device and the real part of Clausius-Mossotti factor of the cells was estimated from critical voltages for particle trapping at the electrodes under steady fluid flow. The dielectric properties of the cell compartments (cytoplasm and membrane) were calculated based on a single shell model of the cells. The separation of C. muris and G. lambia is achieved successfully at a frequency of 10 MHz and a voltage of 3 Vpp (peak to peak voltage). © 2012 American Institute of Physics.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1063/1.3671065
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.contributor.departmentMECHANICAL ENGINEERING
dc.description.doi10.1063/1.3671065
dc.description.sourcetitleBiomicrofluidics
dc.description.volume6
dc.description.issue1
dc.description.page-
dc.identifier.isiut000302301000007
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