Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.biortech.2012.08.085
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dc.titleCharacterization of a butanol-acetone-producing Clostridium strain and identification of its solventogenic genes
dc.contributor.authorChua, T.K.
dc.contributor.authorLiang, D.-W.
dc.contributor.authorQi, C.
dc.contributor.authorYang, K.-L.
dc.contributor.authorHe, J.
dc.date.accessioned2014-06-17T05:29:12Z
dc.date.available2014-06-17T05:29:12Z
dc.date.issued2013-05
dc.identifier.citationChua, T.K., Liang, D.-W., Qi, C., Yang, K.-L., He, J. (2013-05). Characterization of a butanol-acetone-producing Clostridium strain and identification of its solventogenic genes. Bioresource Technology 135 : 372-378. ScholarBank@NUS Repository. https://doi.org/10.1016/j.biortech.2012.08.085
dc.identifier.issn09608524
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/58981
dc.description.abstractA unique Clostridium species strain G117 was obtained in this study to be capable of producing dominant butanol from glucose. Butanol of 13.50. g/L was produced when culture G117 was fed with 60. g/L glucose, which is ~20% higher than previously reported butanol production by wild-type Clostridium acetobutylicum ATCC 824 under similar conditions. Strain G117 also distinguishes itself by generating negligible amount of ethanol, but producing butanol and acetone as biosolvent end-products. A butanol dehydrogenase gene (bdh gene) was identified in strain G117, which demonstrated a ~200-fold increase in transcription level measured by quantitative real-time PCR after 10. h of culture growth. The high transcription suggests that this bdh gene could be a putative gene involved in butanol production. In all, Clostridium sp. strain G117 serves as a potential candidate for industrial biobutanol production while the absence of ethanol ensures an economic-efficient separation and purification of butanol. © 2012 Elsevier Ltd.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.biortech.2012.08.085
dc.sourceScopus
dc.subjectBiofuel
dc.subjectButanol
dc.subjectButanol dehydrogenase
dc.subjectClostridium
dc.subjectSolventogenic
dc.typeArticle
dc.contributor.departmentCIVIL & ENVIRONMENTAL ENGINEERING
dc.contributor.departmentDIVISION OF ENVIRONMENTAL SCIENCE & ENGG
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.description.doi10.1016/j.biortech.2012.08.085
dc.description.sourcetitleBioresource Technology
dc.description.volume135
dc.description.page372-378
dc.description.codenBIRTE
dc.identifier.isiut000319181000052
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