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Title: Characterization of a butanol-acetone-producing Clostridium strain and identification of its solventogenic genes
Authors: Chua, T.K. 
Liang, D.-W. 
Qi, C.
Yang, K.-L. 
He, J. 
Keywords: Biofuel
Butanol dehydrogenase
Issue Date: May-2013
Citation: Chua, T.K., Liang, D.-W., Qi, C., Yang, K.-L., He, J. (2013-05). Characterization of a butanol-acetone-producing Clostridium strain and identification of its solventogenic genes. Bioresource Technology 135 : 372-378. ScholarBank@NUS Repository.
Abstract: A unique Clostridium species strain G117 was obtained in this study to be capable of producing dominant butanol from glucose. Butanol of 13.50. g/L was produced when culture G117 was fed with 60. g/L glucose, which is ~20% higher than previously reported butanol production by wild-type Clostridium acetobutylicum ATCC 824 under similar conditions. Strain G117 also distinguishes itself by generating negligible amount of ethanol, but producing butanol and acetone as biosolvent end-products. A butanol dehydrogenase gene (bdh gene) was identified in strain G117, which demonstrated a ~200-fold increase in transcription level measured by quantitative real-time PCR after 10. h of culture growth. The high transcription suggests that this bdh gene could be a putative gene involved in butanol production. In all, Clostridium sp. strain G117 serves as a potential candidate for industrial biobutanol production while the absence of ethanol ensures an economic-efficient separation and purification of butanol. © 2012 Elsevier Ltd.
Source Title: Bioresource Technology
ISSN: 09608524
DOI: 10.1016/j.biortech.2012.08.085
Appears in Collections:Staff Publications

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