Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.bios.2008.06.055
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dc.titleSpatially well-defined binary brushes of poly(ethylene glycol)s for micropatterning of active proteins on anti-fouling surfaces
dc.contributor.authorXu, F.J.
dc.contributor.authorLi, H.Z.
dc.contributor.authorLi, J.
dc.contributor.authorTeo, Y.H.E.
dc.contributor.authorZhu, C.X.
dc.contributor.authorKang, E.T.
dc.contributor.authorNeoh, K.G.
dc.date.accessioned2014-06-17T03:06:26Z
dc.date.available2014-06-17T03:06:26Z
dc.date.issued2008-12-01
dc.identifier.citationXu, F.J., Li, H.Z., Li, J., Teo, Y.H.E., Zhu, C.X., Kang, E.T., Neoh, K.G. (2008-12-01). Spatially well-defined binary brushes of poly(ethylene glycol)s for micropatterning of active proteins on anti-fouling surfaces. Biosensors and Bioelectronics 24 (4) : 773-780. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bios.2008.06.055
dc.identifier.issn09565663
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/57460
dc.description.abstractWe report a novel method for micropatterning of active proteins on anti-fouling surfaces via spatially well-defined and dense binary poly(ethylene glycol)s (PEGs) brushes with controllable protein-docking sites. Binary brushes of poly(poly(ethylene glycol) methacrylate-co-poly(ethylene glycol)methyl ether methacrylate), or P(PEGMA-co-PEGMEMA), and poly(poly(ethylene glycol)methyl ether methacrylate), or P(PEGMEMA), were prepared via consecutive surface-initiated atom transfer radical polymerizations (SI-ATRPs) from a resist-micropatterned Si(1 0 0) wafer surface. The terminal hydroxyl groups on the side chains of PEGMA units in the P(PEGMA-co-PEGMEMA) microdomains were activated directly by 1,1′-carbonyldiimidazole (CDI) for the covalent coupling of human immunoglobulin (IgG) (as a model active protein). The resulting IgG-coupled PEG microdomains interact only and specifically with target anti-IgG, while the other PEG microregions effectively prevent specific and non-specific protein fouling. When extended to other active biomolecules, microarrays for specific and non-specific analyte interactions with a high signal-to-noise ratio could be readily tailored. © 2008 Elsevier B.V. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.bios.2008.06.055
dc.sourceScopus
dc.subjectATRP
dc.subjectBiosensor
dc.subjectMicropatterning
dc.subjectPEG
dc.subjectProtein
dc.typeArticle
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.contributor.departmentBIOENGINEERING
dc.contributor.departmentELECTRICAL & COMPUTER ENGINEERING
dc.description.doi10.1016/j.bios.2008.06.055
dc.description.sourcetitleBiosensors and Bioelectronics
dc.description.volume24
dc.description.issue4
dc.description.page773-780
dc.description.codenBBIOE
dc.identifier.isiut000261262000042
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