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https://doi.org/10.1016/j.bios.2008.06.055
Title: | Spatially well-defined binary brushes of poly(ethylene glycol)s for micropatterning of active proteins on anti-fouling surfaces | Authors: | Xu, F.J. Li, H.Z. Li, J. Teo, Y.H.E. Zhu, C.X. Kang, E.T. Neoh, K.G. |
Keywords: | ATRP Biosensor Micropatterning PEG Protein |
Issue Date: | 1-Dec-2008 | Citation: | Xu, F.J., Li, H.Z., Li, J., Teo, Y.H.E., Zhu, C.X., Kang, E.T., Neoh, K.G. (2008-12-01). Spatially well-defined binary brushes of poly(ethylene glycol)s for micropatterning of active proteins on anti-fouling surfaces. Biosensors and Bioelectronics 24 (4) : 773-780. ScholarBank@NUS Repository. https://doi.org/10.1016/j.bios.2008.06.055 | Abstract: | We report a novel method for micropatterning of active proteins on anti-fouling surfaces via spatially well-defined and dense binary poly(ethylene glycol)s (PEGs) brushes with controllable protein-docking sites. Binary brushes of poly(poly(ethylene glycol) methacrylate-co-poly(ethylene glycol)methyl ether methacrylate), or P(PEGMA-co-PEGMEMA), and poly(poly(ethylene glycol)methyl ether methacrylate), or P(PEGMEMA), were prepared via consecutive surface-initiated atom transfer radical polymerizations (SI-ATRPs) from a resist-micropatterned Si(1 0 0) wafer surface. The terminal hydroxyl groups on the side chains of PEGMA units in the P(PEGMA-co-PEGMEMA) microdomains were activated directly by 1,1′-carbonyldiimidazole (CDI) for the covalent coupling of human immunoglobulin (IgG) (as a model active protein). The resulting IgG-coupled PEG microdomains interact only and specifically with target anti-IgG, while the other PEG microregions effectively prevent specific and non-specific protein fouling. When extended to other active biomolecules, microarrays for specific and non-specific analyte interactions with a high signal-to-noise ratio could be readily tailored. © 2008 Elsevier B.V. All rights reserved. | Source Title: | Biosensors and Bioelectronics | URI: | http://scholarbank.nus.edu.sg/handle/10635/57460 | ISSN: | 09565663 | DOI: | 10.1016/j.bios.2008.06.055 |
Appears in Collections: | Staff Publications |
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