Please use this identifier to cite or link to this item: https://doi.org/10.1021/la800016h
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dc.titleColloidal crystals from surface-tension-assisted self-assembly: A novel matrix for single-molecule experiments
dc.contributor.authorWen, C.Y.
dc.contributor.authorKannan, B.
dc.contributor.authorWohland, T.
dc.contributor.authorNg, V.
dc.date.accessioned2014-06-17T02:41:46Z
dc.date.available2014-06-17T02:41:46Z
dc.date.issued2008-11-04
dc.identifier.citationWen, C.Y., Kannan, B., Wohland, T., Ng, V. (2008-11-04). Colloidal crystals from surface-tension-assisted self-assembly: A novel matrix for single-molecule experiments. Langmuir 24 (21) : 12142-12149. ScholarBank@NUS Repository. https://doi.org/10.1021/la800016h
dc.identifier.issn07437463
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/55322
dc.description.abstractIn this work, we develop a new method of creating colloidal crystals with cavities for the entrapment and long-term observation of single biomolecules. Colloidal crystals are first fabricated using surface-tension-assisted self-assembly. Surface tension helps to reduce the interparticle distance between dispensed colloids. Subsequently, the colloids are used as a matrix in which single fluorescently tagged molecules can be tracked using fluorescence microscopy. This method has a high efficiency of self-assembly for small volumes (4 μL) of colloidal suspensions (polystyrene colloids with diameters of 1000, 500, 200, and 100 nm) at low concentration (1% w/w). The spatial hindrance effect on the diffusion of molecules and their entrapment is discussed on the basis of fluorescence correlation spectroscopy results from the diffusion of molecules with different hydrodynamic radii in the cavities of colloidal crystals formed from micrometer- to nanometer-sized polystyrene spheres. Single horseradish peroxidase molecules turning over fluorescent products are tracked over a few seconds. This shows that colloidal crystals can be used to test the function of single molecules of enzymes and protein under controlled spatial confinement. © 2008 American Chemical Society.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1021/la800016h
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentCHEMISTRY
dc.contributor.departmentELECTRICAL & COMPUTER ENGINEERING
dc.description.doi10.1021/la800016h
dc.description.sourcetitleLangmuir
dc.description.volume24
dc.description.issue21
dc.description.page12142-12149
dc.description.codenLANGD
dc.identifier.isiut000260508800013
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