Please use this identifier to cite or link to this item: https://doi.org/10.3109/15419061.2012.665968
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dc.title3D coupling of fibronectin fibril arrangement with topology of ventral plasma membrane
dc.contributor.authorHirata, H.
dc.contributor.authorLim, C.T.
dc.contributor.authorMiyata, H.
dc.date.accessioned2014-06-16T09:22:49Z
dc.date.available2014-06-16T09:22:49Z
dc.date.issued2012-04
dc.identifier.citationHirata, H., Lim, C.T., Miyata, H. (2012-04). 3D coupling of fibronectin fibril arrangement with topology of ventral plasma membrane. Cell Communication and Adhesion 19 (2) : 17-23. ScholarBank@NUS Repository. https://doi.org/10.3109/15419061.2012.665968
dc.identifier.issn15419061
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/53859
dc.description.abstractInteraction of integrins with extracellular matrices is essential for cell adhesion to substrata. Ventral surfaces of fibroblasts adhering to flat substrata are not flat but have uneven 3D topology. However, spatial relationship between the topology of the ventral cell surface and arrangement of extracellular matrix fibrils remains unclear. Here, we report a novel and simple method based on total internal reflection fluorescence microscopy to quantify the distance between the ventral plasma membrane and the glass substratum. We observe that the distance varies from <25 nm at focal adhesions to 40-50 nm at close contacts and >80 nm in other regions. Furthermore, by applying this novel method, we show that fibronectin fibrils are also separated from the substratum in regions where the ventral cell surface-substratum distance is >80 nm. Our results reveal that fibronectin fibrils are not simply adsorbed to the glass substratum but follow the ventral cell surface topology. © 2012 Informa Healthcare USA, Inc.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.3109/15419061.2012.665968
dc.sourceScopus
dc.subjectfibronectin
dc.subjectfocal adhesion
dc.subjectintegrin
dc.subjectmembrane topology
dc.subjectplasma membrane
dc.subjecttotal internal reflection fluorescence microscopy
dc.typeArticle
dc.contributor.departmentBIOENGINEERING
dc.description.doi10.3109/15419061.2012.665968
dc.description.sourcetitleCell Communication and Adhesion
dc.description.volume19
dc.description.issue2
dc.description.page17-23
dc.description.codenCCAEB
dc.identifier.isiut000304601900001
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