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Title: Near-Infrared Confocal Raman Spectroscopy for Real-Time Diagnosis of Cervical Precancer
Keywords: cervical precancer, Raman spectroscopy, real-time diagnosis, hormonal variations, in vivo diagnosis, feature selection
Issue Date: 23-Aug-2013
Citation: SHIYAMALA DURAIPANDIAN (2013-08-23). Near-Infrared Confocal Raman Spectroscopy for Real-Time Diagnosis of Cervical Precancer. ScholarBank@NUS Repository.
Abstract: Near-infrared (NIR) Raman spectroscopy is a vibrational spectroscopic technique capable of non-destructively probing endogenous biomolecules and their changes associated with tissue disease transformation. In this dissertation, we develop a real-time, portable, cost-effective, integrated fingerprint (FP) and high wavenumber (HW) confocal Raman spectroscopy for simultaneous acquisition of FP and HW Raman spectra predominantly from the epithelial layer of cervix to enhance cervical precancer diagnosis in vivo. We further established the Raman diagnostic framework integrated with the developed system, enabling the colposcopists? to perform routine point-wise scanning for targeted biopsies of high-risk tissue sites in sub-seconds. Firstly, we realized the in vitro Raman spectroscopic diagnosis of cervical cancer at the cellular level and further characterized different stages of cervical tissue precancer (i.e., low-grade and high-grade) with promising results. We actualized that the diagnosis achieved using in vivo confocal Raman diagnostic modality was superior to NIR autofluorescence (AF) spectroscopy, but marginally higher than the co contributions of NIR AF and confocal Raman spectroscopy. The confocal Raman spectroscopy in conjunction with feature selection algorithm (e.g., genetic algorithm (GA)) identifies the diagnostically important Raman features to further improve the diagnosis of cervical precancer in vivo. We also demonstrate for the first time the promising potential of HW confocal Raman spectroscopy in conjunction with biomolecular modeling for identifying hormone/menopause-related variations in native squamous epithelium of normal cervix, as well as for assessing the effect of Vagifem treatment on postmenopausal atrophic cervix in vivo during clinical colposcopic inspections. We also evidenced the diagnostic utility of FP and HW confocal Raman spectroscopy alone and in combination for identifying compositional changes in cervix associated with precancer transformation. The study disclosed that the complementary information obtained from FP and HW confocal Raman spectroscopy can enhance the in vivo diagnosis of cervical precancer. It is expected that the integrated FP/HW confocal Raman spectroscopy coupled with the on-line spectroscopic diagnostic framework has the potential to become a promising clinical diagnostic tool in adjunct to colposcopy for realizing real-time in vivo diagnosis of cervical precancer.
Appears in Collections:Ph.D Theses (Open)

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