Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.jviromet.2004.01.002
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dc.titleIn situ hybridization of a marine fish virus, Singapore grouper iridovirus with a nucleic acid probe of major capsid protein
dc.contributor.authorHuang, C.
dc.contributor.authorZhang, X.
dc.contributor.authorGin, K.Y.H.
dc.contributor.authorQin, Q.W.
dc.date.accessioned2014-05-20T02:29:39Z
dc.date.available2014-05-20T02:29:39Z
dc.date.issued2004-05
dc.identifier.citationHuang, C., Zhang, X., Gin, K.Y.H., Qin, Q.W. (2004-05). In situ hybridization of a marine fish virus, Singapore grouper iridovirus with a nucleic acid probe of major capsid protein. Journal of Virological Methods 117 (2) : 123-128. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jviromet.2004.01.002
dc.identifier.issn01660934
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/53448
dc.description.abstractA DNA probe of 531 base pairs for Singapore grouper iridovirus (SGIV) was generated by polymerase chain reaction and labeled with nonradioactive digoxigenin. An in situ hybridization based method was developed to detect SGIV in formalin-fixed tissues from maricultured Malabar grouper, Epinephelus malabaricus Bloch and Schneider. The in situ hybridization detected SGIV in the kidney, spleen, liver, intestine, stomach and gills from naturally infected fish. Strong hybridization signals were obtained from the kidney and spleen tissues, while intermediate intensity signals were observed in the intestine and liver tissues. The weakest signals were obtained from the stomach and gills. The signals were located specifically within epithelial, endothelial and sub-endothelial hypertrophic cells in all tested tissues. The in situ hybridization procedure will provide an important diagnostic tool to complement histopathological methods, and contribute to epidemiological studies on the origin and distribution of iridovirus in mariculture. © 2004 Elsevier B.V. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.jviromet.2004.01.002
dc.sourceScopus
dc.subjectDNA probe
dc.subjectGrouper
dc.subjectIn situ hybridization
dc.subjectIridovirus
dc.typeArticle
dc.contributor.departmentTROPICAL MARINE SCIENCE INSTITUTE
dc.contributor.departmentNATIONAL UNIVERSITY MEDICAL INSTITUTES
dc.description.doi10.1016/j.jviromet.2004.01.002
dc.description.sourcetitleJournal of Virological Methods
dc.description.volume117
dc.description.issue2
dc.description.page123-128
dc.description.codenJVMED
dc.identifier.isiut000220770200003
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