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Title: Analysis of recombinant human tumor necrosis factor beta by capillary electrophoresis
Authors: Yao, Y.J.
Loh, K.C. 
Chung, M.C.M.
Li, S.F.Y. 
Keywords: capillary electrophoresis
recombinant-DNA technology
tumor necrosis factor
Issue Date: 1995
Citation: Yao, Y.J.,Loh, K.C.,Chung, M.C.M.,Li, S.F.Y. (1995). Analysis of recombinant human tumor necrosis factor beta by capillary electrophoresis. Electrophoresis 16 (4) : 647-653. ScholarBank@NUS Repository.
Abstract: Capillary electrophoresis was used for the analysis of recombinnt tumor necrosis factor beta (rhTNF-β). Reproducible separation of the protein could be achieved in Polybrene-coated capillaries. Optimum conditions for separation were achieved with a pH 4.5 sodium acetate buffer. Besides Polybrene-coated capillaries, other commercially available column and column-coating reagents were also examined for the separation of rhTNF-β. Capillary electrophoresis was found to be a potentially useful method for product analysis and process monitoring in recombinant-DNA technology.
Source Title: Electrophoresis
ISSN: 01730835
Appears in Collections:Staff Publications

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