Please use this identifier to cite or link to this item: https://doi.org/10.1007/s13238-010-0020-3
DC FieldValue
dc.titleThe class A macrophage scavenger receptor type I (SR-AI) recognizes complement iC3b and mediates NF-κB activation
dc.contributor.authorGoh, J.W.K.
dc.contributor.authorTan, Y.S.
dc.contributor.authorDodds, A.W.
dc.contributor.authorReid, K.B.M.
dc.contributor.authorLu, J.
dc.date.accessioned2014-05-19T02:55:43Z
dc.date.available2014-05-19T02:55:43Z
dc.date.issued2010-02
dc.identifier.citationGoh, J.W.K., Tan, Y.S., Dodds, A.W., Reid, K.B.M., Lu, J. (2010-02). The class A macrophage scavenger receptor type I (SR-AI) recognizes complement iC3b and mediates NF-κB activation. Protein and Cell 1 (2) : 174-187. ScholarBank@NUS Repository. https://doi.org/10.1007/s13238-010-0020-3
dc.identifier.issn1674800X
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/53215
dc.description.abstractThe macrophage scavenger receptor SR-AI binds to host tissue debris to perform clearance and it binds to bacteria for phagocytosis. In addition, SR-AI modulates macrophage activation through cell signaling. However, investigation of SR-AI signaling on macrophages is complicated due to its promiscuous ligand specificity that overlaps with other macrophage receptors. Therefore, we expressed SR-AI on HEK 293T cells to investigate its ligand binding and signaling. On 293Tcells, SR-AI could respond to E. coli DH5α, leading to NF-κB activation and IL-8 production. However, this requires E. coli DH5α to be sensitized by fresh serum that is treated with heat-inactivation or complement C3 depletion. Anti-C3 antibody inhibits the binding of SR-AI to serum-sensitized DH5α and blocks DH5α stimulation of SR-AI signaling. Further analysis showed that SR-AI can directly bind to purified iC3b but not C3 or C3b. By mutagenesis, The SRCR domain of SR-AI was found to be essential in SR-AI binding to serum-sensitized DH5α. These results revealed a novel property of SR-AI as a complement receptor for iC3b-opsonized bacteria that can elicit cell signaling. © 2010 Higher Education Press and Springer-Verlag Berlin Heidelberg.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1007/s13238-010-0020-3
dc.sourceScopus
dc.subject293T cells
dc.subjectcomplement
dc.subjectiC3b
dc.subjectmacrophage
dc.subjectsignalling
dc.subjectSR-AI
dc.typeArticle
dc.contributor.departmentMICROBIOLOGY
dc.contributor.departmentZOOLOGY
dc.description.doi10.1007/s13238-010-0020-3
dc.description.sourcetitleProtein and Cell
dc.description.volume1
dc.description.issue2
dc.description.page174-187
dc.identifier.isiut000208510400010
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

14
checked on Aug 18, 2022

WEB OF SCIENCETM
Citations

14
checked on Aug 11, 2022

Page view(s)

161
checked on Aug 18, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.