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Title: Neural stem cell specific fluorescent chemical probe binding to FABP7
Authors: Yun, S.-W.
Leong, C.
Zhai, D.
Tan, Y.L.
Lim, L.
Bi, X.
Lee, J.-J.
Kim, H.J. 
Kang, N.-Y.
Ng, S.H.
Stanton, L.W.
Chang, Y.-T. 
Keywords: BLBP
High throughput screening
Mass spectrometry
Radial glia
Issue Date: 26-Jun-2012
Citation: Yun, S.-W., Leong, C., Zhai, D., Tan, Y.L., Lim, L., Bi, X., Lee, J.-J., Kim, H.J., Kang, N.-Y., Ng, S.H., Stanton, L.W., Chang, Y.-T. (2012-06-26). Neural stem cell specific fluorescent chemical probe binding to FABP7. Proceedings of the National Academy of Sciences of the United States of America 109 (26) : 10214-10217. ScholarBank@NUS Repository.
Abstract: Fluorescent small molecules have become indispensable tools for biomedical research along with the rapidly developing optical imaging technology. We report here a neural stem cell specific borondipyrromethane (BODIPY) derivative compound of designation red 3 (CDr3), developed through a high throughput/content screening of in-house generated diversity oriented fluorescence library in stem cells at different developmental stages. This novel compound specifically detects living neural stem cells of both human and mouse origin. Furthermore, we identified its binding target by proteomic analysis as fatty acid binding protein 7 (FABP7), also known as brain lipid binding protein) which is highly expressed in neural stem cells and localized in the cytoplasm. CDr3 will be a valuable chemical tool in the study and applications of neural stem cells.
Source Title: Proceedings of the National Academy of Sciences of the United States of America
ISSN: 00278424
DOI: 10.1073/pnas.1200817109
Appears in Collections:Staff Publications

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