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Title: Ice nucleation inhibition: Mechanism of antifreeze by antifreeze protein
Authors: Du, N. 
Liu, X.Y. 
Hew, C.L. 
Issue Date: 19-Sep-2003
Citation: Du, N., Liu, X.Y., Hew, C.L. (2003-09-19). Ice nucleation inhibition: Mechanism of antifreeze by antifreeze protein. Journal of Biological Chemistry 278 (38) : 36000-36004. ScholarBank@NUS Repository.
Abstract: The effect of antifreeze protein type III (one type of fish antifreeze protein) on ice crystallization was examined quantitatively based on a "micro-sized ice nucleation" technique. It was found for the first time that antifreeze proteins can inhibit the ice nucleation process by adsorbing onto both the surfaces of ice nuclei and dust particles. This leads to an increase of the ice nucleation barrier and the desolvation kink kinetics barrier, respectively. Based on the latest nucleation model, the increases in the ice nucleation barrier and the kink kinetics barrier were measured. This enables us to quantitatively examine the antifreeze mechanism of antifreeze proteins for the first time.
Source Title: Journal of Biological Chemistry
ISSN: 00219258
DOI: 10.1074/jbc.M305222200
Appears in Collections:Staff Publications

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