Please use this identifier to cite or link to this item: https://doi.org/10.1002/bit.24822
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dc.titleA thin-walled polydimethylsiloxane bioreactor for high-density hepatocyte sandwich culture
dc.contributor.authorTan, G.-D.S.
dc.contributor.authorToh, G.W.
dc.contributor.authorBirgersson, E.
dc.contributor.authorRobens, J.
dc.contributor.authorvan Noort, D.
dc.contributor.authorLeo, H.L.
dc.date.accessioned2014-05-16T04:58:07Z
dc.date.available2014-05-16T04:58:07Z
dc.date.issued2013-06
dc.identifier.citationTan, G.-D.S., Toh, G.W., Birgersson, E., Robens, J., van Noort, D., Leo, H.L. (2013-06). A thin-walled polydimethylsiloxane bioreactor for high-density hepatocyte sandwich culture. Biotechnology and Bioengineering 110 (6) : 1663-1673. ScholarBank@NUS Repository. https://doi.org/10.1002/bit.24822
dc.identifier.issn00063592
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/52510
dc.description.abstractIn vitro drug testing requires long-term maintenance of hepatocyte liver specific functions. Hepatocytes cultured at a higher seeding density in a sandwich configuration exhibit an increased level of liver specific functions when compared to low density cultures due to the better cell to cell contacts that promote long term maintenance of polarity and liver specific functions. However, culturing hepatocytes at high seeding densities in a standard 24-well plate poses problems in terms of the mass transport of nutrients and oxygen to the cells. In view of this drawback, we have developed a polydimethylsiloxane (PDMS) bioreactor that was able to maintain the long-term liver specific functions of a hepatocyte sandwich culture at a high seeding density. The bioreactor was fabricated with PDMS, an oxygen permeable material, which allowed direct oxygenation and perfusion to take place simultaneously. The mass transport of oxygen and the level of shear stress acting on the cells were analyzed by computational fluid dynamics (CFD). The combination of both direct oxygenation and perfusion has a synergistic effect on the liver specific function of a high density hepatocyte sandwich culture over a period of 9 days. © 2012 Wiley Periodicals, Inc.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1002/bit.24822
dc.sourceScopus
dc.subjectDiffusion
dc.subjectDirect oxygenation
dc.subjectMass transport
dc.subjectOxygen concentration
dc.subjectShear stress
dc.typeArticle
dc.contributor.departmentBIOENGINEERING
dc.contributor.departmentMECHANOBIOLOGY INSTITUTE
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.description.doi10.1002/bit.24822
dc.description.sourcetitleBiotechnology and Bioengineering
dc.description.volume110
dc.description.issue6
dc.description.page1663-1673
dc.description.codenBIBIA
dc.identifier.isiut000318107800013
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