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Title: A thin-walled polydimethylsiloxane bioreactor for high-density hepatocyte sandwich culture
Authors: Tan, G.-D.S.
Toh, G.W.
Birgersson, E. 
Robens, J. 
van Noort, D.
Leo, H.L. 
Keywords: Diffusion
Direct oxygenation
Mass transport
Oxygen concentration
Shear stress
Issue Date: Jun-2013
Citation: Tan, G.-D.S., Toh, G.W., Birgersson, E., Robens, J., van Noort, D., Leo, H.L. (2013-06). A thin-walled polydimethylsiloxane bioreactor for high-density hepatocyte sandwich culture. Biotechnology and Bioengineering 110 (6) : 1663-1673. ScholarBank@NUS Repository.
Abstract: In vitro drug testing requires long-term maintenance of hepatocyte liver specific functions. Hepatocytes cultured at a higher seeding density in a sandwich configuration exhibit an increased level of liver specific functions when compared to low density cultures due to the better cell to cell contacts that promote long term maintenance of polarity and liver specific functions. However, culturing hepatocytes at high seeding densities in a standard 24-well plate poses problems in terms of the mass transport of nutrients and oxygen to the cells. In view of this drawback, we have developed a polydimethylsiloxane (PDMS) bioreactor that was able to maintain the long-term liver specific functions of a hepatocyte sandwich culture at a high seeding density. The bioreactor was fabricated with PDMS, an oxygen permeable material, which allowed direct oxygenation and perfusion to take place simultaneously. The mass transport of oxygen and the level of shear stress acting on the cells were analyzed by computational fluid dynamics (CFD). The combination of both direct oxygenation and perfusion has a synergistic effect on the liver specific function of a high density hepatocyte sandwich culture over a period of 9 days. © 2012 Wiley Periodicals, Inc.
Source Title: Biotechnology and Bioengineering
ISSN: 00063592
DOI: 10.1002/bit.24822
Appears in Collections:Staff Publications

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