Please use this identifier to cite or link to this item:
|Title:||Synchrotron radiation-induced formation and reactions of free radicals in human acellular dermal matrix||Authors:||Gouk, S.-S.
|Issue Date:||May-2005||Citation:||Gouk, S.-S., Kocherginsky, N.M., Kostetski, Y.Y., Moser, H.O., Yang, P., Lim, T.-M., Sun, W.Q. (2005-05). Synchrotron radiation-induced formation and reactions of free radicals in human acellular dermal matrix. Radiation Research 163 (5) : 535-543. ScholarBank@NUS Repository. https://doi.org/10.1667/RR3351||Abstract:||The present work characterizes the formation of free radicals in an implantable human acellular dermal tissue (Alloderm®, LifeCell Corp., Branchburg, NJ) upon irradiation. The tissue was preserved in a vitreous carbohydrate matrix by freeze-drying. Freeze-dried samples were irradiated using a synchrotron light source, and free radicals generated were investigated using the electron paramagnetic resonance (EPR) technique. At least two free radical populations, with g factors of 1.993 (∼43%) and 2.002 (∼57%), respectively, were identified in the irradiated tissue. The transformation (reaction) kinetics of free radicals produced was investigated in the presence of nitrogen, oxygen and moisture. The reaction kinetics of free radicals was extremely slow in the nitrogen environment. The presence of oxygen and moisture greatly accelerated free radical reactions in the tissue matrix. The reaction of free radicals could not be described by traditional reaction kinetics. A dispersive kinetics model and a diffusion model were developed to analyze the reaction kinetics in the present study. The dispersive model took into consideration molecular mobility and dispersivity of free radicals in the heterogeneous tissue material. The diffusion model described the radical reaction kinetics as two parallel and simultaneous processes: a first-order fast kinetics mainly on tissue surface and a diffusion-limited slow kinetics in deeper layers of the tissue matrix. Both models described quantitative experimental data well. Further investigation is needed to verify whether any of these two models or concepts describes the inherent radical reaction kinetics in the solid tissue matrix. © 2005 by Radiation Research Society.||Source Title:||Radiation Research||URI:||http://scholarbank.nus.edu.sg/handle/10635/51822||ISSN:||00337587||DOI:||10.1667/RR3351|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.