Please use this identifier to cite or link to this item:
Title: Osteogenic differentiation of human Wharton's jelly stem cells on nanofibrous substrates in vitro
Authors: Gauthaman, K.
Venugopal, J.R. 
Yee, F.C.
Biswas, A.
Ramakrishna, S. 
Bongso, A.
Issue Date: 1-Jan-2011
Citation: Gauthaman, K., Venugopal, J.R., Yee, F.C., Biswas, A., Ramakrishna, S., Bongso, A. (2011-01-01). Osteogenic differentiation of human Wharton's jelly stem cells on nanofibrous substrates in vitro. Tissue Engineering - Part A 17 (1-2) : 71-81. ScholarBank@NUS Repository.
Abstract: Most tissue engineering studies use human bone marrow mesenchymal stem cells for differentiation into desirable lineages. We derived a novel stem cell from the human umbilical cord Wharton's jelly (hWJSC) that has numerous advantages over other stem cell types in that they can be harvested in abundance very efficiently and painlessly with no risk of patient morbidity, have prolonged stemness properties in vitro, are hypoimmunogenic, and can be differentiated into many tissue types in two-dimensional culture. We compared four different three-dimensional nanofibrous scaffolds (polycaprolactone [PCL], PCL/collagen [PCL/Coll], PCL/hydroxyapatite [PCL/HA], and PCL/Coll/HA) for the attachment, proliferation, differentiation, and mineralization of hWJSCs into an osteogenic lineage. The collagen-based scaffolds (PCL/Coll and PCL/Coll/HA) showed better cell attachment and proliferation than PCL and PCL/HA, with increases of 41.80% and 38.52%, respectively. hWJSCs cultured on PCL/Coll/HA in the osteogenic medium up to 21 days demonstrated increased alkaline phosphatase activity and greater expression of osteocalcin, mineralization, and osteogenic-related genes compared to controls. Given the advantages of hWJSCs over other stem cell types, we propose that hWJSCs may be efficiently differentiated into an osteogenic lineage on a three-dimensional PCL/Coll/HA nanofibrous scaffold for the treatment of bone defects. © 2011 Mary Ann Liebert, Inc.
Source Title: Tissue Engineering - Part A
ISSN: 19373341
DOI: 10.1089/ten.tea.2010.0224
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Oct 20, 2021


checked on Oct 20, 2021

Page view(s)

checked on Oct 14, 2021

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.