Fabrication of 3D chitosan-hydroxyapatite scaffolds using a robotic dispensing system

Abstract

A new robotic desktop rapid prototyping (RP) system was designed to fabricate scaffolds for tissue engineering applications. The experimental setup consists of a computer-guided desktop robot and a one-component pneumatic dispenser. The dispensing material (chitosan and chitosan-hydroxyapatite (HA) dissolved in acetic acid) was stored in a 30-ml barrel and forced out through a small Teflonlined nozzle into a dispensing medium (sodium hydroxide-ethanol in ratio of 7:3). Layer-by-layer, the chitosan was fabricated with a preprogramed lay-down pattern. Neutralization of the chitosan forms a gel-like precipitate, and the hydrostatic pressure in the sodium hydroxide (NaOH) solution keeps the cuboid scaffold in shape. Comparison of the freeze-dried scaffold to the wet one showed linear and volumetric shrinkage of about 31% and 62%, respectively. A good attachment between layers allowed the chitosan matrix to form a fully interconnected channel architecture. Results of in vitro cell culture studies revealed the scaffold biocompatibility. The results of this preliminary study using the rapid prototyping robotic dispensing (RPBOD) system demonstrated its potential in fabricating three-dimensional (3D) scaffolds with regular and reproducible macropore architecture. © 2002 Elsevier Science B.V. All rights reserved.