Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0928-4931(02)00014-0
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dc.titleCharacterization of anterior cruciate ligament cells and bone marrow stromal cells on various biodegradable polymeric films
dc.contributor.authorOuyang, H.W.
dc.contributor.authorGoh, J.C.H.
dc.contributor.authorMo, X.M.
dc.contributor.authorTeoh, S.H.
dc.contributor.authorLee, E.H.
dc.date.accessioned2014-04-24T09:31:36Z
dc.date.available2014-04-24T09:31:36Z
dc.date.issued2002-05-31
dc.identifier.citationOuyang, H.W., Goh, J.C.H., Mo, X.M., Teoh, S.H., Lee, E.H. (2002-05-31). Characterization of anterior cruciate ligament cells and bone marrow stromal cells on various biodegradable polymeric films. Materials Science and Engineering C 20 (1-2) : 63-69. ScholarBank@NUS Repository. https://doi.org/10.1016/S0928-4931(02)00014-0
dc.identifier.issn09284931
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/51349
dc.description.abstractIn this study, the adhesion, proliferation and morphology of rabbit anterior cruciate ligament (ACL) cells and bone marrow stromal cells (bMSCs) on synthetic biodegradable polymeric films were investigated. Tissue culture polystyrene (TCP) was used as control. Seven biodegradable polymers were used; they are as follows: poly(ε-caprolactone) (PCL), poly(DL-lactide) (D-PLA), poly(L-lactide) (L-PLA), PLA/PCL (50:50), PLA/PCL (75:25), high molecular weight (HMW) poly(DL-lactide-co-glycolide (PLGA50:50) and HMW PLGA75:25. Polymeric film substrates were manufactured using solvent spin-casting technique. After 8 h of cell culture, a high percentage of ACL cells was found attached to PLGA50:50 (38.6 ± 8.4%) and TCP (39.3 ± 6.1%) as compared to the other six polymeric films (p ≤ 0.001). As for bMSCs, 76.4 ± 10%, 76.3 ± 16% and 76.1 ± 19% of seeded bMSCs were adhered to TCP, PLGA50:50 and PLGA75:25, respectively. These were significantly more than those of the other five polymeric films (p < 0.001). At Day 5, bMSCs were found to proliferate faster on TCP (by 7 ± 0.8-fold of initial cell seeding number), D-PLA (by 5.6 ± 1.6-fold), PLGA50:50 (by 9.3 ± 1.3-fold) and PLGA75:25 (by 5.8 ± 1.3-fold) than on PCL, PLLA and PCL/PLA (50:50, 25:75) (p < 0.001). ACL cells had a greater fold expansion on TCP (by 3.5 ± 0.2-fold), PLGA50:50 (by 3.1 ± 0.4-fold) and PLGA75:25 (by 3.9 ± 0.4-fold) than on the other five polymer substrates (p < 0.001). From these results, HMW PLGA (50:50, 75:25) was shown more likely to allow bMSCs and ACL cells to attach and proliferate, and bMSCs attached and proliferated faster than ACL cells. © 2002 Elsevier Science B.V. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0928-4931(02)00014-0
dc.sourceScopus
dc.subjectACL
dc.subjectBiodegradable polymers
dc.subjectbMSCs
dc.subjectCell adhesion
dc.subjectCell morphology
dc.subjectCell proliferation
dc.typeArticle
dc.contributor.departmentMECHANICAL ENGINEERING
dc.contributor.departmentORTHOPAEDIC SURGERY
dc.description.doi10.1016/S0928-4931(02)00014-0
dc.description.sourcetitleMaterials Science and Engineering C
dc.description.volume20
dc.description.issue1-2
dc.description.page63-69
dc.identifier.isiut000176088200010
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