Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.msec.2010.06.004
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dc.titleBio-functionalized PCL nanofibrous scaffolds for nerve tissue engineering
dc.contributor.authorGhasemi-Mobarakeh, L.
dc.contributor.authorPrabhakaran, M.P.
dc.contributor.authorMorshed, M.
dc.contributor.authorNasr-Esfahani, M.H.
dc.contributor.authorRamakrishna, S.
dc.date.accessioned2014-04-24T09:31:22Z
dc.date.available2014-04-24T09:31:22Z
dc.date.issued2010-10-12
dc.identifier.citationGhasemi-Mobarakeh, L., Prabhakaran, M.P., Morshed, M., Nasr-Esfahani, M.H., Ramakrishna, S. (2010-10-12). Bio-functionalized PCL nanofibrous scaffolds for nerve tissue engineering. Materials Science and Engineering C 30 (8) : 1129-1136. ScholarBank@NUS Repository. https://doi.org/10.1016/j.msec.2010.06.004
dc.identifier.issn09284931
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/51342
dc.description.abstractSurface properties of scaffolds such as hydrophilicity and the presence of functional groups on the surface of scaffolds play a key role in cell adhesion, proliferation and migration. Different modification methods for hydrophilicity improvement and introduction of functional groups on the surface of scaffolds have been carried out on synthetic biodegradable polymers, for tissue engineering applications. In this study, alkaline hydrolysis of poly (ε-caprolactone) (PCL) nanofibrous scaffolds was carried out for different time periods (1 h, 4 h and 12 h) to increase the hydrophilicity of the scaffolds. The formation of reactive groups resulting from alkaline hydrolysis provides opportunities for further surface functionalization of PCL nanofibrous scaffolds. Matrigel was attached covalently on the surface of an optimized 4 h hydrolyzed PCL nanofibrous scaffolds and additionally the fabrication of blended PCL/matrigel nanofibrous scaffolds was carried out. Chemical and mechanical characterization of nanofibrous scaffolds were evaluated using attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy, contact angle, scanning electron microscopy (SEM) and tensile measurement. In vitro cell adhesion and proliferation study was carried out after seeding nerve precursor cells (NPCs) on different scaffolds. Results of cell proliferation assay and SEM studies showed that the covalently functionalized PCL/matrigel nanofibrous scaffolds promote the proliferation and neurite outgrowth of NPCs compared to PCL and hydrolyzed PCL nanofibrous scaffolds, providing suitable substrates for nerve tissue engineering. © 2010 Elsevier B.V.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.msec.2010.06.004
dc.sourceScopus
dc.subjectAlkaline hydrolysis
dc.subjectMatrigel
dc.subjectNanofibrous scaffolds
dc.subjectNerve tissue engineering
dc.subjectPCL
dc.typeArticle
dc.contributor.departmentNUS NANOSCIENCE & NANOTECH INITIATIVE
dc.contributor.departmentMECHANICAL ENGINEERING
dc.description.doi10.1016/j.msec.2010.06.004
dc.description.sourcetitleMaterials Science and Engineering C
dc.description.volume30
dc.description.issue8
dc.description.page1129-1136
dc.identifier.isiut000282905600008
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