Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.snb.2005.04.018
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dc.titleSimultaneous cell lysis and bead trapping in a continuous flow microfluidic device
dc.contributor.authorRamadan, Q.
dc.contributor.authorSamper, V.
dc.contributor.authorPoenar, D.
dc.contributor.authorLiang, Z.
dc.contributor.authorYu, C.
dc.contributor.authorLim, T.M.
dc.date.accessioned2014-04-23T07:09:15Z
dc.date.available2014-04-23T07:09:15Z
dc.date.issued2006-02-27
dc.identifier.citationRamadan, Q., Samper, V., Poenar, D., Liang, Z., Yu, C., Lim, T.M. (2006-02-27). Simultaneous cell lysis and bead trapping in a continuous flow microfluidic device. Sensors and Actuators, B: Chemical 113 (2) : 944-955. ScholarBank@NUS Repository. https://doi.org/10.1016/j.snb.2005.04.018
dc.identifier.issn09254005
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/50735
dc.description.abstractContinuous flow cell lysis by electroporation (EP), and cell and bead trapping by dielectrophoresis (DEP) have been demonstrated on a dielectrophoretic microfluidic chip. The results of lysing human white blood cells (WBC) and murine clonal cells (MN9D) by electroporation showed that lysis rates of up to 80% were achieved at 3 MHz and a flow rate of 30 μl/min. It was also shown that the lysis reactor can function as a dielelectrophoretic filter for trapping silica beads which selectively bind to DNA. The combination of cell lysis, DNA binding to beads, and bead separation assay in a single structure offers a step forward in the development of an efficient and compact system for fully automated microfluidic DNA sample preparation. The continuous flow protocol addresses major challenges faced in sample preparation such as lysate clogging, cell sedimentation, and cell agglomeration. © 2005 Elsevier B.V. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.snb.2005.04.018
dc.sourceScopus
dc.subjectCell lysis
dc.subjectContinuous flow
dc.subjectDielectrophoresis
dc.subjectDNA extraction
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.contributor.departmentCIVIL ENGINEERING
dc.description.doi10.1016/j.snb.2005.04.018
dc.description.sourcetitleSensors and Actuators, B: Chemical
dc.description.volume113
dc.description.issue2
dc.description.page944-955
dc.description.codenSABCE
dc.identifier.isiut000235593200049
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