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Title: Interventions Into Ageing Using Methods Targeted at the Mitochondria in the C. Elegans Model
Keywords: Mitochondria, Peroxiredoxin-3, Reactive Oxygen Species, Nematodes, Developmentally Normal
Issue Date: 2-Aug-2013
Citation: MANICKARATNAM RANJAN (2013-08-02). Interventions Into Ageing Using Methods Targeted at the Mitochondria in the C. Elegans Model. ScholarBank@NUS Repository.
Abstract: The mitochondrial free radical theory proposes that ageing is due to an accumulation of oxidative damage caused by reactive oxygen species that are generated during metabolism. Modulating the rate of oxidative damage accumulation is therefore expected to alter the rate of ageing. However, studies attempting to test this by modulating antioxidant defences have reported conflicting results. In mammals prdx-3 is predicted to detoxify 90% of mitochondrial hydrogen peroxide. Here we investigate the effects of repressed prdx-3 expression in developmentally normal wild type C. elegans. We found evidence that in the absence of normal prdx-3 mediated antioxidant defences, ROS homeostasis was maintained by mild mitochondrial uncoupling. Consequently, net ROS production, oxidative protein damage accumulation, and lifespan remained normal. Mild mitochondrial uncoupling also reduced ATP availability, leading to reduced physical and reproductive fitness. We discuss the implications of our data for the mitochondrial free radical theory of ageing.
Appears in Collections:Ph.D Theses (Open)

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