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Title: Development of an encapsulation technique in control of pathogen
Keywords: Influenza A H5N1 virus, Vaccine delivery, Hemagglutinin (HA), Microencapsulation, Electrostatic extrusion, Alginate matrix
Issue Date: 4-May-2006
Citation: MOU HONGHUI (2006-05-04). Development of an encapsulation technique in control of pathogen. ScholarBank@NUS Repository.
Abstract: Influenza A H5N1 virus demonstrated its capacity to directly infect humans in Hong Kong in 1997, and succeeded again in Viet Nam in January 2004. Vaccination remains the principal measure for preventing influenza and reducing the impact of epidemics. This study investigated the use of microencapsulation in alginate matrix for antigen delivery. Bovine serum albumin (BSA) and partial purified hemagglutinin (HA) proteins were encapsulated in alginate matrix by electrostatic extrusion to form a delivery system which allowed for controlled release. We found out that applied voltage, flow rate, electrode spacing and needle size played theirs roles in microcapsule formation, and models were established between microcapsule size and two dominant factors, i.e., applied voltage and flow rate. Meanwhile, protein release was found affected by microcapsule size, pH, protein loading, and release medium. The release would also be significantly delayed if chitosan coating was applied on the Ca-alginate microcapsules.
Appears in Collections:Master's Theses (Open)

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