Please use this identifier to cite or link to this item:
https://scholarbank.nus.edu.sg/handle/10635/47645
DC Field | Value | |
---|---|---|
dc.title | KERATIN REMODELING IN STRESS | |
dc.contributor.author | TAN TONG SAN | |
dc.date.accessioned | 2013-11-11T18:01:29Z | |
dc.date.available | 2013-11-11T18:01:29Z | |
dc.date.issued | 2012-08-13 | |
dc.identifier.citation | TAN TONG SAN (2012-08-13). KERATIN REMODELING IN STRESS. ScholarBank@NUS Repository. | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/47645 | |
dc.description.abstract | Remodeling of keratin cytoskeletal network is essential for cells to respond to environmental cues. To understand the stress/wound response, immortalized keratinocytes are used which expressed a keratin mutation mimicking severe EBS Dowling-Meara (a skin fragility disorder), fluorescently tagged for live-cell imaging. All keratinocytes are ¿activated¿ to a stress state through up-regulation of kinases and wound response proteins, but revert to quiescence after confluence. In mutant cells, ¿activation¿ is reflected by the presence of keratin aggregates, a hallmark of severe EBS-DM. It is thought that avoiding aggregate formation may improve the disease phenotype. It is observed that EGF influences keratin aggregate formation/dynamics in mutant keratinocytes, through changes in plectin expression, during cell migration. Phosphorylation at K14 Y129 is found to play a role in keratin aggregate formation, modulating cell migration during scratch wound assays. This thesis demonstrates how plasticity of the keratin cytoskeleton can modulate the skin cell¿s response to environmental cues. | |
dc.language.iso | en | |
dc.subject | Keratin remodeling, epidermolysis bullosa simplex, wound response, EGF, keratin phosphorylation, cell migration | |
dc.type | Thesis | |
dc.contributor.department | NUS GRAD SCH FOR INTEGRATIVE SCI & ENGG | |
dc.contributor.supervisor | ELLEN BIRGITTE LANE | |
dc.description.degree | Ph.D | |
dc.description.degreeconferred | DOCTOR OF PHILOSOPHY | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Ph.D Theses (Open) |
Show simple item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
TanTS.pdf | 5.72 MB | Adobe PDF | OPEN | None | View/Download |
Google ScholarTM
Check
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.