Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/47616
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dc.titleREGULATION OF C1Q AT TRANSCRIPTIONAL LEVEL
dc.contributor.authorTAN SHURONG CAROL
dc.date.accessioned2013-11-11T18:00:36Z
dc.date.available2013-11-11T18:00:36Z
dc.date.issued2012-08-02
dc.identifier.citationTAN SHURONG CAROL (2012-08-02). REGULATION OF C1Q AT TRANSCRIPTIONAL LEVEL. ScholarBank@NUS Repository.
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/47616
dc.description.abstractThe complement protein C1q is a large assembly from six subunits each of C1qA, C1qB and C1qC. The subunit genes are highly clustered, and any mutation in the genes can cause genetic C1q deficiency. To understand the synchronized, macrophage/dendritic cell-specific transcription of C1q genes, the three mouse C1q promoters were characterized, but only C1qb promoter displayed bona fide IFN-¿-stimulated activity. Cloning C1qb promoter at the 3¿-end supplemented 5¿ C1qa and C1qc promoter activities. The basal activity of each C1q promoter required a functional PU.1 site, and C1qb promoter depended on an EICE-ISRE overlapping element for IFN-¿ response. Ectopic PU.1 expression in non-myeloid HEK 293T cells conferred activities to C1q promoters. In BMDCs, the C1q gene locus assumed a unique conformation that brought C1q promoters into close spatial proximity, possibly contributing to the coordinated C1q gene expression in macrophage/dendritic cells. A transgenic BAC vector was constructed to generate transgenic mice.
dc.language.isoen
dc.subjectC1q, Chromosome conformation capture, BMDC, Transcription, PU.1, IFN-γ
dc.typeThesis
dc.contributor.departmentNUS GRAD SCH FOR INTEGRATIVE SCI & ENGG
dc.contributor.supervisorLU JINHUA
dc.description.degreePh.D
dc.description.degreeconferredDOCTOR OF PHILOSOPHY
dc.identifier.isiutNOT_IN_WOS
Appears in Collections:Ph.D Theses (Open)

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