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Title: Electrochemical DNA sensor for detection of environmental pathogens
Authors: VARUN RAI
Keywords: Electrochemistry, Signal amplification, DNA biosensors, Legionella sp, DENV
Issue Date: 3-Jan-2013
Citation: VARUN RAI (2013-01-03). Electrochemical DNA sensor for detection of environmental pathogens. ScholarBank@NUS Repository.
Abstract: Concept of electrochemical signal amplification has been extended to develop an ultrasensitive electrochemical amplified molecular beacon biosensor to detect target DNA sequence of Legionella sp. An electrochemically amplified molecular beacon (EAMB) biosensor is constructed using thiolated hairpin DNA-ferrocene probes on gold electrode based on electrochemical signal amplification strategy to detect target DNA sequences of Legionella pneumophila. Ultrasensitive DNA detection can be achieved either amplifying sensing signal, suppressing background noise, utilizing nanomaterial based nanoporous structure for attaching probe molecules to develop sensing surface. We have also developed DNA biosensors based on nanoporous alumina membrane to detect target DNA strands of Legionella sp and cDNA detection of (DENV) dengue virus RNA. The above mentioned biosensors are also tested against longer strands of ssDNA sample derived from genomic sequences of Legionella pneumophila and dengue virus using asymmetric PCR methods.
Appears in Collections:Ph.D Theses (Open)

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