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Title: Laser scanning confocal microscopy and laser tweezers based experiments to understand dentine-bacteria interactions
Authors: Sum, C.
Mohanty, S.
Gupta, P.K.
Kishen, A. 
Keywords: Bacteria adhesion
Calcium hydroxide
Confocal microscopy
Enterococcus faecalis
Laser tweezers
Type-I collagen
Issue Date: 2007
Citation: Sum, C., Mohanty, S., Gupta, P.K., Kishen, A. (2007). Laser scanning confocal microscopy and laser tweezers based experiments to understand dentine-bacteria interactions. Progress in Biomedical Optics and Imaging - Proceedings of SPIE 6425 : -. ScholarBank@NUS Repository.
Abstract: Failure of endodontic treatment is commonly due to Enterococcal infection. In this study influence of chemical treatments of type-I collagen membrane by chemical agents commonly used in endodontic treatment on Enterococcus faecalis cell adherence was evaluated. In order to determine the change in number of adhering bacteria after chemical treatment, confocal laser scanning microscopy was used. For this, overnight culture of E faecalis in All Culture broth was applied to chemically treated type-I collagen membrane. It was found that Ca(OH)2 treated groups had statistically significant (p value=0.05) increase in population of bacteria adherence. The change in adhesion force between bacteria and collagen was determined by using optical tweezers (1064 nm). For this experiment, Type-I collagen membrane was soaked for 5 mins in a media that contained 50% all culture media and 50% saturated Ca(OH)2. The membrane was spread on the coverslip, on which diluted bacterial suspension was added. The force of laser tweezers on the bacteria was estimated at different trap power levels using viscous drag method and trapping stiffness was calculated using Equipartition theorem method. Presence of Ca(OH)2 was found to increase the cell-substrate adherence force from 0.38pN to >2.1pN. Together, these experiments show that it was highly probable that the increase in adherence to collagen was due to a stronger adhesion in the presence of Ca (OH)2.
Source Title: Progress in Biomedical Optics and Imaging - Proceedings of SPIE
ISBN: 0819465380
ISSN: 16057422
DOI: 10.1117/12.701784
Appears in Collections:Staff Publications

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