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Title: Imaging carious dental tissues with multiphoton fluorescence lifetime imaging microscopy
Authors: Lin, P.-Y.
Lyu, H.-C.
Hsu, C.-Y.S. 
Chang, C.-S.
Kao, F.-J.
Issue Date: 2011
Citation: Lin, P.-Y., Lyu, H.-C., Hsu, C.-Y.S., Chang, C.-S., Kao, F.-J. (2011). Imaging carious dental tissues with multiphoton fluorescence lifetime imaging microscopy. Biomedical Optics Express 2 (1) : 149-158. ScholarBank@NUS Repository.
Abstract: In this study, multiphoton excitation was utilized to image normal and carious dental tissues noninvasively. Unique structures in dental tissues were identified using the available multimodality (second harmonic, autofluorescence, and fluorescence lifetime analysis) without labeling. The collagen in dentin exhibits a strong second harmonic response. Both dentin and enamel emit strong autofluorescence that reveals in detail morphological features (such as dentinal tubules and enamel rods) and, despite their very similar spectral profiles, can be differentiated by lifetime analysis. Specifically, the carious dental tissue exhibits a greatly reduced autofluorescence lifetime, which result is consistent with the degree of demineralization, determined by micro-computed tomography. Our findings suggest that two-photon excited fluorescence lifetime imaging may be a promising tool for diagnosing and monitoring dental caries. © 2010 Optical Society of America.
Source Title: Biomedical Optics Express
ISSN: 21567085
DOI: 10.1364/BOE.2.000149
Appears in Collections:Staff Publications

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