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https://doi.org/10.1089/ten.tec.2010.0735
DC Field | Value | |
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dc.title | Establishment of clinically compliant human embryonic stem cells in an autologous feeder-free system | |
dc.contributor.author | Fu, X. | |
dc.contributor.author | Toh, W.S. | |
dc.contributor.author | Liu, H. | |
dc.contributor.author | Lu, K. | |
dc.contributor.author | Li, M. | |
dc.contributor.author | Cao, T. | |
dc.date.accessioned | 2013-10-16T05:19:31Z | |
dc.date.available | 2013-10-16T05:19:31Z | |
dc.date.issued | 2011 | |
dc.identifier.citation | Fu, X., Toh, W.S., Liu, H., Lu, K., Li, M., Cao, T. (2011). Establishment of clinically compliant human embryonic stem cells in an autologous feeder-free system. Tissue Engineering - Part C: Methods 17 (9) : 927-937. ScholarBank@NUS Repository. https://doi.org/10.1089/ten.tec.2010.0735 | |
dc.identifier.issn | 19373384 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/46592 | |
dc.description.abstract | Applications of human embryonic stem cells (hESCs) are limited by the use of mouse embryonic fibroblasts feeder and animal-derived components during culture. In this study, we demonstrated the potential use of extracellular matrix (ECM) derived from the autologous feeders to support long-term undifferentiated growth of hESCs in xeno-free, serum-free, and feeder-free conditions. Autologous H9 ebF (feeder cells derived from outgrowth of embryoid body [EB] predifferentiated from H9 hESCs) was derived from EBs predifferentiated from H9 hESCs through a direct-plating outgrowth system. The ECM comprising collagen VI, laminin, and fibronectin was extracted from H9 ebF through a freeze-thaw procedure. The autologous ECM together with animal component-free TeSR™2 medium was used to support long-term growth of H1 and H9 hESC lines for up to 20 passages. The maintenance of hESC undifferentiated state by autologous ECM was confirmed by the positive staining of hESC-specific markers (Oct4, SSEA-4, and Tra-1-60) and the expression of pluripotency marker genes (Oct4, Nanog, and Sox2). Flow cytometry further showed that more than 99% of hESCs retained the expression of SSEA-3/4 after long-term culture on autologous ECM. Pluripotency of hESCs on ECM was further proven by in vitro EB formation and in vivo teratoma assay. Overall, this study suggested a strategy for efficient propagation of clinically compliant hESCs in an autologous feeder-free culture system. © 2011 Mary Ann Liebert, Inc. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1089/ten.tec.2010.0735 | |
dc.source | Scopus | |
dc.type | Article | |
dc.contributor.department | ORAL AND MAXILLOFACIAL SURGERY | |
dc.description.doi | 10.1089/ten.tec.2010.0735 | |
dc.description.sourcetitle | Tissue Engineering - Part C: Methods | |
dc.description.volume | 17 | |
dc.description.issue | 9 | |
dc.description.page | 927-937 | |
dc.identifier.isiut | 000294701400006 | |
Appears in Collections: | Staff Publications |
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