Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/42965
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dc.titleINTERLEUKIN-6 RELEASE FROM T98G HUMAN GLIAL CELL LINE AS A PREDICTIVE MARKER FOR CHRONIC PAIN, AND THE CHARACTERIZATION OF SUBSTANCE(S) INVOLVED IN PAIN
dc.contributor.authorTAY SUAN ANNABEL
dc.date.accessioned2013-07-16T18:00:13Z
dc.date.available2013-07-16T18:00:13Z
dc.date.issued2013-01-08
dc.identifier.citationTAY SUAN ANNABEL (2013-01-08). INTERLEUKIN-6 RELEASE FROM T98G HUMAN GLIAL CELL LINE AS A PREDICTIVE MARKER FOR CHRONIC PAIN, AND THE CHARACTERIZATION OF SUBSTANCE(S) INVOLVED IN PAIN. ScholarBank@NUS Repository.
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/42965
dc.description.abstractThis work focuses on pro-inflammatory cytokine release in a cell culture system as a potential marker for chronic pain. Cerebrospinal fluid (CSF) from post-herpetic neuralgia (PHN) and osteroarthritic patients was used to trigger T98G cell cultures. Interleukin (IL-6) release in chronic pain patient groups was significantly higher than that of pain-free controls, and in the patient groups whose treatment was ineffective. CSF samples collected pre-treatment also triggered higher IL-6 than post-treatment samples. Secondly, fractions in chronic pain patients¿ CSF that triggered IL-6 release in T98G cells were isolated. They would be subjected to future mass spectrometry analysis to identify protein-like substances in CSF which could be potential chronic pain markers. Lastly, western blot analysis to elucidate the IL-6 signaling pathway showed NF-¿B acting upstream of IL-6. This in vitro assay system quantifying chronic pain and the understanding of the complex mechanisms could contribute to the future management of unrelenting pain.
dc.language.isoen
dc.subjectChronic pain, Postherpetic Neuralgia, Osteoarthritis, Interleukin-6, Cerebrospinal fluid, NF-kB
dc.typeThesis
dc.contributor.departmentANAESTHESIA
dc.contributor.supervisorLOW CHIAN MING
dc.contributor.supervisorLIU HERN CHOON, EUGENE
dc.contributor.supervisorLEE TAT LEANG
dc.description.degreeMaster's
dc.description.degreeconferredMASTER OF SCIENCE
dc.identifier.isiutNOT_IN_WOS
Appears in Collections:Master's Theses (Open)

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