Please use this identifier to cite or link to this item:
Title: CTCF-mediated functional chromatin interactome in pluripotent cells
Authors: Handoko, L.
Xu, H.
Li, G.
Ngan, C.Y.
Chew, E.
Schnapp, M.
Lee, C.W.H.
Ye, C.
Ping, J.L.H.
Mulawadi, F.
Wong, E.
Sheng, J.
Zhang, Y.
Poh, T.
Chan, C.S.
Kunarso, G.
Shahab, A.
Bourque, G.
Cacheux-Rataboul, V.
Sung, W.-K. 
Ruan, Y.
Wei, C.-L.
Issue Date: 2011
Citation: Handoko, L., Xu, H., Li, G., Ngan, C.Y., Chew, E., Schnapp, M., Lee, C.W.H., Ye, C., Ping, J.L.H., Mulawadi, F., Wong, E., Sheng, J., Zhang, Y., Poh, T., Chan, C.S., Kunarso, G., Shahab, A., Bourque, G., Cacheux-Rataboul, V., Sung, W.-K., Ruan, Y., Wei, C.-L. (2011). CTCF-mediated functional chromatin interactome in pluripotent cells. Nature Genetics 43 (7) : 630-638. ScholarBank@NUS Repository.
Abstract: Mammalian genomes are viewed as functional organizations that orchestrate spatial and temporal gene regulation. CTCF, the most characterized insulator-binding protein, has been implicated as a key genome organizer. However, little is known about CTCF-associated higher-order chromatin structures at a global scale. Here we applied chromatin interaction analysis by paired-end tag (ChIA-PET) sequencing to elucidate the CTCF-chromatin interactome in pluripotent cells. From this analysis, we identified 1,480 cis- and 336 trans-interacting loci with high reproducibility and precision. Associating these chromatin interaction loci with their underlying epigenetic states, promoter activities, enhancer binding and nuclear lamina occupancy, we uncovered five distinct chromatin domains that suggest potential new models of CTCF function in chromatin organization and transcriptional control. Specifically, CTCF interactions demarcate chromatin-nuclear membrane attachments and influence proper gene expression through extensive cross-talk between promoters and regulatory elements. This highly complex nuclear organization offers insights toward the unifying principles that govern genome plasticity and function. © 2011 Nature America, Inc. All rights reserved.
Source Title: Nature Genetics
ISSN: 10614036
DOI: 10.1038/ng.857
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Oct 22, 2021


checked on Oct 22, 2021

Page view(s)

checked on Oct 14, 2021

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.