Please use this identifier to cite or link to this item:
https://doi.org/10.1142/S0219720012310026
Title: | Tutorial on de novo peptide sequencing using MS/MS mass spectrometry | Authors: | Chong, K.F. Leong, H.W. |
Keywords: | algorithms De novo peptide sequencing spectrum graph survey tandem mass spectrometry tutorial |
Issue Date: | 2012 | Citation: | Chong, K.F., Leong, H.W. (2012). Tutorial on de novo peptide sequencing using MS/MS mass spectrometry. Journal of Bioinformatics and Computational Biology 10 (6). ScholarBank@NUS Repository. https://doi.org/10.1142/S0219720012310026 | Abstract: | This paper is a self-contained introductory tutorial on the problem in proteomics known as peptide sequencing using tandem mass spectrometry. This tutorial deals specifically with de novo sequencing methods (as opposed to database search methods). We first give an introduction to peptide sequencing, its importance and history and some background on proteins. Next we show the relationship between a peptide and the final spectrum produced from a tandem mass spectrometer, together with a description of the various sources of complications that arise during the process of generating the mass spectrum. From there we model the computational problem of de novo peptide sequencing, which is basically the reverse problem of identifying the peptide which produced the spectrum. We then present several major approaches to solve it (including reviewing some of the current algorithms in each approach), and also discuss related problems and post-processing approaches. © 2012 Imperial College Press. | Source Title: | Journal of Bioinformatics and Computational Biology | URI: | http://scholarbank.nus.edu.sg/handle/10635/39135 | ISSN: | 02197200 | DOI: | 10.1142/S0219720012310026 |
Appears in Collections: | Staff Publications |
Show full item record
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.