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|Title:||MECHANOBIOLOGY OF NANOTOPOGRAPHY INDUCED DIFFERENTIATION IN HUMAN MESENCHYMAL STEM CELL||Authors:||TEO KIM KIAT||Keywords:||Mesenchymal stem cell, cell signaling, FAK, differentiation, focal adhesions, physical cues||Issue Date:||24-Jan-2013||Citation:||TEO KIM KIAT (2013-01-24). MECHANOBIOLOGY OF NANOTOPOGRAPHY INDUCED DIFFERENTIATION IN HUMAN MESENCHYMAL STEM CELL. ScholarBank@NUS Repository.||Abstract:||Biophysical cues, such as nanotopography, have been shown to be integral for tissue regeneration and embryogenesis in the stem cell niche. Tissue homeostasis involves the interaction of multipotent cells with nano-scaled topographical features in their ECM to regulate aspects of cell behaviors. Synthetic nanostructures can drive specific cell differentiation but the sensing mechanism of nanocues remains poorly understood. This project seeks to use engineered extracellular matrix in the form of substrate nanotopography to elucidate the roles of focal adhesion kinase (FAK) and actin cytoskeletal contractility in the nanotopography-induced differentiation of human mesenchymal stem cells (hMSCs). In addition, nuclear changes in hMSCs due to nanotopography-induced differentiation were also investigated. Quantitative flow cytometry results showed significant changes in histone methylation (H3K9me1) and nuclear matrix protein Lamin A upon hMSC exposure to nanotopography, suggesting an influence of nanotopography on nuclear gene regulatory mechanism.||URI:||http://scholarbank.nus.edu.sg/handle/10635/38644|
|Appears in Collections:||Ph.D Theses (Open)|
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