Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.gene.2004.09.024
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dc.titleCloning and molecular characterization of the first aquatic hyaluronidase, SFHYA1, from the venom of stonefish (Synanceja horrida)
dc.contributor.authorNg, H.C.
dc.contributor.authorRanganathan, S.
dc.contributor.authorChua, K.L.
dc.contributor.authorKhoo, H.E.
dc.date.accessioned2013-06-05T09:50:17Z
dc.date.available2013-06-05T09:50:17Z
dc.date.issued2005
dc.identifier.citationNg, H.C., Ranganathan, S., Chua, K.L., Khoo, H.E. (2005). Cloning and molecular characterization of the first aquatic hyaluronidase, SFHYA1, from the venom of stonefish (Synanceja horrida). Gene 346 : 71-81. ScholarBank@NUS Repository. https://doi.org/10.1016/j.gene.2004.09.024
dc.identifier.issn03781119
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/38304
dc.description.abstractWe report here for the first time the molecular characterization of a hyaluronidase from an aquatic source. SFHYA1 is the hyaluronidase found in the venom gland of stonefish, Synanceja horrida. Using a cDNA segment amplified with degenerate oligonucleotides based on the amino acid sequences of a conserved region in testicular-type hyaluronidases and a tryptic fragment of SFHYA1, clones encoding the precursor of this enzyme were isolated from a cDNA library prepared from stonefish venom glands. The deduced amino acid sequence of SFHYA1 shows that SFHYA1 is expressed as a precursor peptide with a 28-residue signal peptide for targeting it into endoplasmic reticulum. Mature SFHYA1 is a polypeptide composed of 449 residues containing three potential N-glycosylation sites, four putative hyaluronan-binding motifs [B(X)7B] and various residues implicated in substrate binding and catalysis. This cDNA was expressed in an active form in insect-cells but not in E. coli. Homology-based computational analyses suggested that SFHYA1 closely resembles the PH-20 family of hyaluronidases. © 2004 Elsevier B.V. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.gene.2004.09.024
dc.sourceScopus
dc.subjectcDNA
dc.subjectHyaluronidases
dc.subjectPH-20
dc.subjectStonefish
dc.typeArticle
dc.contributor.departmentBIOCHEMISTRY
dc.description.doi10.1016/j.gene.2004.09.024
dc.description.sourcetitleGene
dc.description.volume346
dc.description.page71-81
dc.description.codenGENED
dc.identifier.isiut000227900000009
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